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相关概念视频

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

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Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
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Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.0K
Epigenetic Regulation01:37

Epigenetic Regulation

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Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.0K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

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Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
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Position-effect Variegation02:32

Position-effect Variegation

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In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
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相关实验视频

Updated: Jun 24, 2025

A Method to Study de novo Formation of Chromatin Domains
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靠近终端产生一个转录状态,决定了Polycomb沉默的建立速度.

Govind Menon1, Eduardo Mateo-Bonmati2, Svenja Reeck2

  • 1Department of Computational and Systems Biology, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.

Molecular cell
|June 8, 2024
PubMed
概括

聚镇压复合体2 (PRC2) 沉默装置由转录合镇压控制. 协同转录处理调节生产转录,决定了Polycomb抑制复合体2 (PRC2) 沉默的速度.

关键词:
H3K27me3 在线阅读H3K4me1 的意思是什么?聚合体沉声器是一种聚合体沉声器.替代性的多基化.模拟和数字基因调节.共同转录的处理.有关反,反的互动.机械学数学建模 机械学数学建模接近端的终点.转录的对抗性 转录的对抗性

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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

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相关实验视频

Last Updated: Jun 24, 2025

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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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科学领域:

  • 表观遗传学和转录的调节.
  • 植物分子生物学 植物分子生物学
  • 染色体的动态 染色体的动态

背景情况:

  • 由于Polycomb抑制复合体2 (PRC2) 的新染色体介导的转录沉默的机制和时间表尚不清楚.
  • 阿拉比多普西斯中的FLOWERING LOCUS C (FLC) 基因作为研究PRC2沉默的模型,涉及RNA处理,基因组脱甲基化和PRC2活性.

研究的目的:

  • 为了研究共同转录RNA处理,基因素修饰和PRC2-介导的转录沉默在Arabidopsis FLC位点之间的机械联系.
  • 开发和验证一个计算模型,解释转录合抑制如何影响建立PRC2沉默.

主要方法:

  • 开发和测试一个计算模型,整合近接多基解/终结 (通过FCA),H3K4me1去除 (通过FLD) 和RNA聚合酶II (RNA Pol II) 进程性.
  • 关于共转录处理在调节转录和PRC2沉默动态中的作用的模型预测的实验验证.

主要成果:

  • 开发了一个计算模型,描述了FCA中介终止如何诱导FLD依赖的H3K4me1删除.
  • 证明H3K4me1的去除可以降低RNA Pol II的过程性,增强早期终止和抑制生产性转录.
  • 模型预测证实,转录合的抑制水平决定了建立PRC2/H3K27me3沉默的时间表.

结论:

  • 在FLC位置的协同转录RNA处理确定了生产转录的水平.
  • 这种生产性转录水平直接影响过渡到Polycomb压制复合体2 (PRC2) 中介沉默的速度.
  • 该研究阐明了一种新的反机制,将RNA处理与基于染色体的转录抑制联系起来.