车辆和灌解决方案和方法对从根管中去除双抗生素糊的影响:来自高性能液体染色学分析的见解
Pornsupa Tuamsomboon1, Suporn Charumanee2, Taepin Junmahasathien2
1Postgraduate Student in Endodontics, Faculty of Dentistry, Department of Restorative Dentistry and Periodontology, Chiang Mai University, Mueang, Chiang Mai, Thailand.
Journal of endodontics
|June 8, 2024
概括
酸有效地从根管中去除抗生素残留物,而基甲基纤维素 (HPMC) 是双抗生素糊 (DAP) 的优越载体,与宏醇和甘醇 (MP) 相比. 高性能液态色谱 (HPLC) 准确量化这些残留物.
科学领域:
- 牙周内科医院 牙周内科医院 牙周内科医院
- 生物材料科学 生物材料科学
- 分析化学 分析化学
背景情况:
- 再生性内牙科需要完全清除抗生素残留物以保护干细胞.
- 之前的研究在量化残留抗生素方面缺乏精度.
- 高性能液体色谱 (HPLC) 为药物检测提供了增强的灵敏度和特异性.
研究的目的:
- 评估不同载体和灌溶液/方法的疗效,以从根管中去除双抗生素糊 (DAP).
- 为了比较宏醇/甘醇 (MP) 和基甲基纤维素 (HPMC) 作为DAP的载体.
- 为了评估酸和乙烯基胺三酸 (EDTA) 灌的有效性,使用不同的输送方法.
主要方法:
- 使用MP或HPMC车辆制备了两种DAP配方 (西普洛克萨和美特罗尼达).
- DAP应用于模拟的不成熟牙,培养28天,然后灌.
- 用HPLC分析灌溶液和牙样本,以量化剩余的抗生素.
主要成果:
- 与EDTA相比,酸在去除抗生素方面显示出显著更高的疗效.
- 灌方法 (正压注射器与被动超声波灌) 对抗生素去除没有显著影响.
- 基于HPMC的DAP促进了比基于MP的DAP更好地清除西普洛素.
结论:
- 酸是一种有效的灌剂,可以从根管中去除抗生素糊.
- 对于DAP配方来说,HPMC是一个比MP更好的载体.
- 在内牙科应用中,HPLC是量化残留抗生素的宝贵工具.
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