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HTR2B作为慢性阻塞性肺病的新生物标志物,具有肺状细胞癌

Yue Li¹, Yu Wang¹, Ruhao Wu¹

¹Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China.

Scientific reports

|June 8, 2024

概括

这项研究确定了HTR2B作为慢性阻塞性肺病 (COPD) 和肺状细胞癌 (LUSC) 的潜在生物标志物和治疗标. 升调HTR2B与慢性肺炎,上皮层-介质细胞过渡和LUSC进展有关.

科学领域:

在瘤学瘤学.
肺部病理学肺部病理学
分子生物学分子生物学

背景情况:

慢性阻塞性肺病 (COPD) 与肺状细胞癌 (LUSC) 有共同的病因因素,包括吸烟,炎症和氧化应激.
在COPD中,在重复损伤和修复过程中进行气道改造可能会导致癌症的发展.
改变的炎症和免疫微环境与COPD和LUSC的恶性转变有关.

研究的目的:

为了确定与LUSC相关的COPD的新生物标志物.
研究HTR2B在COPD中的作用及其作为LUSC治疗点的潜力.

主要方法:

利用GSE76925数据集进行差异基因表达分析.
采用LASSO回归和随机森林模型来识别关键的COPD基因.
使用CIBERSORT分析了免疫细胞组成,并在独立数据集和细胞模型中验证了HTR2B的表达.
在HTR2B操纵后评估了上皮层-介质细胞转换 (EMT) 标记物和LUSC细胞增殖.

主要成果:

在COPD中鉴定了1082个差异表达基因 (DEG),富含p53信号和β-氨酸代谢途径.
拉索和随机森林模型确定了HTR2B,DPYS,FRY和CD19作为与免疫细胞相关的关键COPD基因.
在COPD患者和细胞模型中发现HTR2B的升高调节,与EMT标志物增加相关.
在LUSC细胞中,HTR2B的表达也被上调,其抑制减少了LUSC细胞的增殖.

结论:

HTR2B是一种潜在的COPD新生物标志物.
在COPD患者中,HTR2B可能在LUSC的发病过程中发挥重要作用.
在COPD的背景下,HTR2B代表了LUSC的潜在治疗标.

关键词:

生物标志物生物标志物慢性慢性肺炎是一种慢性慢性肺炎,COPD是一种慢性肺炎.在EMT中,EMT是EMT.在LUSC中使用LUSC.机器学习机器学习

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