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相关概念视频

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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相关实验视频

Updated: Jun 24, 2025

Robust 3D DNA FISH Using Directly Labeled Probes
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多色和谱系特异性相间染色体Flow-FISH:方法开发和临床验证

Huey-En Tzeng1, Yi-Wei Lee2, Chien-Ting Lin3

  • 1Division of Hematology/Medical Oncology, Department of Medicine, Taichung Veterans General Hospital, Taichung City, Taiwan; Ph.D. Program for Cancer Molecular Biology and Drug Discovery, and Graduate Institute of Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan; Department of Post-Baccalaureate Medicine, College of Medicine, National Chung-Hsing University, Taichung, Taiwan.

Pathology
|June 9, 2024
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概括

这项研究引入了一种新的多色流细胞计法,用于同时检测核FISH信号和细胞表面标记物. 这一进步使得临床样本中精确的奇默分析和三症12检测成为可能.

关键词:
鱼类 鱼类 是一种鱼类.流式鱼类 流式鱼类细胞免疫类型的形成.流动细胞计量是流动细胞计量的方法.血统特定的 FISH 鱼类.

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FISH for Pre-implantation Genetic Diagnosis
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FISH for Pre-implantation Genetic Diagnosis

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Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay
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Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay

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Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay
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Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay

Published on: February 6, 2012

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科学领域:

  • 细胞测量和遗传学
  • 分子生物学分子生物学
  • 临床诊断 临床诊断 临床诊断

背景情况:

  • 跨相染色体 (IC) 流动-FISH协议仅限于单色检测.
  • 在FISH中DNA变性破坏了细胞完整性,阻碍了并发的表面标记分析.
  • 现有的方法很难将多色FISH与流细胞计结合起来,以进行全面的细胞分析.

研究的目的:

  • 开发一个协议,用于同时多色流细胞计检测核IC FISH信号和细胞表面标记物.
  • 为了验证血造后干细胞移植患者中嵌合体状况的协议.
  • 在慢性淋巴细胞白血病 (CLL) 中适应检测三症12的方法.

主要方法:

  • 开发了一种用于多色IC Flow-FISH的新方案,与表面标记标签相结合.
  • 使用性染色体分析验证了该协议,以检测血液细胞中的嵌合体.
  • 在CLL患者样本中应用了检测三形12的方法.

主要成果:

  • 在检测多种颜色和并发的核/表面信号方面表现出高度一致性.
  • 在56个临床样本中实现了与常规FISH进行模拟分析的高相关性 (R2>0.96).
  • 成功地在B细胞和T细胞中区分了三体病12信号局部化,并在移植后检测出罕见的残留CLL细胞.

结论:

  • 可适应的多色,谱系特定的IC Flow-FISH协议克服了以前的局限性.
  • 这种方法为目前使用传统FISH的各种临床应用提供了潜力.
  • 该技术增强了染色体异常和嵌合体分析的诊断能力.