在废弃活性污泥和肉类加工废弃物的无氧共消化过程中,微生物群体的变化和代谢途径的分析
Fang Cao1, Yuqi Wu1, Longmei Xu1
1College of Environmental Science and Engineering, Taiyuan University of Technology, 209 Daxue Road, Jinzhong, 030600, PR China.
Journal of environmental management
|June 9, 2024
概括
与肉类加工废弃物一起消化废弃物活性污泥显著增加了甲的生产. 这一过程增强了有机降解和生物气生成的关键微生物通路.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 废物活性污泥 (WAS) 和肉类加工废物 (MPW) 是大量的有机废物.
- 无氧共消化 (AcD) 是一个有前途的废物利用和生物气生产技术.
- 优化AcD参数对于最大限度地提高甲产量至关重要.
研究的目的:
- 调查WAS和MPW联合消化的对甲生产的影响.
- 在AcD期间分析微生物社区结构和代谢途径.
- 确定最佳的共同消化比率,以提高生物气体的产生.
主要方法:
- 生物化学甲潜力 (BMP) 测试使用不同比例的WAS和MPW进行.
- 使用16S rRNA高通量测序分析了微生物社区结构.
- 使用测序数据的功能分析预测了代谢途径.
主要成果:
- 与WAS单独相比,联合消化显著增加了甲产量,50%的WAS+50%的MPW比率产生了599.61mL/g的VS.
- 增加MPW改变了微生物群落,增加了像Syntrophomonas和Petrimonas这样的主导细菌,以及像Methanomassiliicoccus和Methanobacterium这样的古生物.
- 合成酸盐氧化 (SAO),性和甲基性甲基生成的关键酶被上调,而乙类甲基生成被抑制.
结论:
- 同时消化WAS和MPW是一个有效的策略,以提高甲生产.
- 增强的甲产量归因于向SAO转变,并与变性和甲基变性甲生成相结合.
- 这项研究提供了对微生物动力学和代谢途径的洞察,以优化无氧消化过程.
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