2+结合将二维双氧化酶的截断的EF手域转移到单体的单体
Chin-Chuan Wei1, Amena Abdul Razzak2, Hadis Ghasemi2
1Department of Chemistry, College of Arts and Sciences, Southern Illinois University Edwardsville, Edwardsville, IL 62026, USA; Department of Pharmaceutical Sciences, College of Pharmacy, Southern Illinois University Edwardsville, Edwardsville, IL 62026, USA.
结合与双氧化酶的结合.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- 过氧化对于甲状腺激素的合成至关重要,由双氧化酶 (Duox) 生产.
- 双氧化酶 (Duox) 激活由 (Ca2+) 与其EF手域 (EFD) 结合来调节.
研究的目的:
- 描述一个截断的Duox EF手域 (EFD) 的Ca2+结合热力学,动力学和形状变化.
主要方法:
- 频谱测量是一种光谱测量.
- 热量测量方法热量测量方法
- 电泳性移动性 电泳性移动性
- 凝过的过方法
- 冷电子显微镜 (Cryo-EM) 是一种电子显微镜.
主要成果:
- 第二个EF手 (EF2) 显示出强大的Ca2+结合 (Ka = 10^7 M^-1),而EF1则表现出较弱的结合 (Ka = 10^5 M^-1).
- 2+结合稳定了EFD结构,将融温度从67°C提高到99°C,并诱导二元转变为单元转变.
- EF2调解了阿波二分体的形成,而与Ca2+结合的EF1的疏水性暴露对全二分体的形成至关重要.
结论:
- Ca2+结合会诱导Duox EFD的显著结构变化,稳定其结构并改变其寡合体状态.
- 结合Ca2+的EFD的稳定结构和暴露的疏水性斑块对于Duox的域间相互作用和电子转移至关重要.
- 这些发现为Duox调节和甲状腺激素合成提供了洞察力.
更多相关视频
14:34Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay
Published on: December 25, 2021
11:27A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
Published on: November 2, 2018
相关概念视频
Cooperative Allosteric Transitions
Electron Transport Chain: Complex III and IV
Structure of Cadherins
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
Allosteric Proteins-ATCase
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
