微粒含有双重亡因子,可以抑制巨细胞的炎症作用
Sean R Simpson1, Denzel D Middleton1, Nicole Rose Lukesh1
1Division of Pharmacoengineering & Molecular Pharmaceutics, Eshelman School of Pharmacy, UNC, Chapel Hill, NC, USA.
Journal of pharmaceutical sciences
|June 9, 2024
概括
装有脂素 (PS) 和酸受体 (AhR) 激动剂的工程微粒显示出通过抑制炎症和恢复免疫耐受性来治疗自身免疫性疾病的前景.
科学领域:
- 免疫学 免疫学 免疫学
- 生物材料科学 生物材料科学
- 药物运输 药物运输 药物运输
背景情况:
- 自身免疫性疾病需要通过免疫耐受机制启发的新型治疗方法.
- 细胞分裂,即亡细胞 (ACs) 的清除,是一个关键的耐受性过程.
- AC利用氨酸 (PS) 暴露和氨酸碳化合物受体 (AhR) 路径激活来诱导耐受性.
研究的目的:
- 开发和评估载有PS和AhR激动剂 (ITE) 的乙化德克斯 (Ace-DEX) 微粒 (MP) 的治疗潜力.
- 在实验室中评估这些装有PS/ITE的MP的免疫调节作用.
主要方法:
- 艾斯-德克斯的MP被装满了PS和/或AhR激动剂ITE.
- 试验室研究涉及培养MPs与腹膜巨细胞 (PMacs),并评估细胞因子的产生 (例如IL-10) 和基因表达 (例如M2标志物,IDO1).
- 评估了抑制脂聚糖 (LPS) 刺激的炎症和T细胞原始化/Th1两极化.
主要成果:
- 装有PS/ITE的Ace-DEXMPs (PS/ITEMPs) 在PMacs中刺激了抗炎细胞因子的表达.
- 在LPS刺激的PMacs中,PS/ITE MP抑制了炎症.
- PS/ITE MPs诱导了IDO1的表达,并抑制了巨细胞介导的T细胞原始化和Th1极化.
结论:
- 装有PS/ITE的Ace-DEXMP在体外显示出显著的抗炎和耐受性特性.
- 这些工程MP有望通过调节免疫反应来治疗自身免疫性疾病的治疗策略.
- 对PS/ITEMP的进一步调查可能会导致对炎症疾病的新治疗方法.
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