流体剪切应力改变了内皮细胞中的克拉斯林动力学和囊泡形成
Tomasz J Nawara1, Jie Yuan1, Leslie D Seeley1
1Department of Cell, Developmental, and Integrative Biology, University of Alabama at Birmingham, Birmingham, Alabama.
Biophysical journal
|June 10, 2024
概括
流体剪切应力 (FSS) 增强了内皮细胞 (ECs) 中的克拉特林介导内细胞分裂 (CME). 这项研究表明,FSS增加了克拉斯林动力学和囊泡形成,使CME适应血液流动条件.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 机械生物学 机械生物学
背景情况:
- 内皮细胞 (ECs) 受到动态机械应力,包括流体剪切应力 (FSS).
- 克拉特林介导的内细胞分裂 (CME) 对于细胞过程至关重要,但在非ECs中可以通过机械应激来抑制.
- 在生理力学力下的EC中CME的适应在很大程度上仍未被探索.
研究的目的:
- 研究生理流体剪切应激 (FSS) 对人静脉内皮细胞 (HUVECs) 中的克拉特林动力学和克拉特林介导内皮细胞形成 (CME) 的影响.
- 为了确定EC是否可以在模仿血液流动的生物物理挑战条件下维持或适应CME.
主要方法:
- 利用同时使用两波长轴比计 (STAR) 显微镜观察内细胞动态.
- 采用DrSTAR数据处理平台,分析在接受生理性FSS的HUVEC中的克拉特林动态.
- 与静态控制器在流动刺激的HUVEC中比较了clathrin动力学.
主要成果:
- 与静态对照组相比,暴露于生理FSS的HUVEC表现出显著增加的克拉特林动力学.
- FSS导致克拉斯林涂层囊泡形成增加了2.3倍,非生产性平坦克拉斯林格子增加了1.9倍.
- 流动刺激的细胞显示曲率启动相对于克拉特林招募的延迟,表明向平到曲线的克拉特林过渡的转变.
结论:
- 克拉的动态和克拉涂层囊泡的形成是由当地的生理环境调节的,特别是EC中的FSS.
- 电子电路根据血液流动的机械线索来适应CME过程,这表明它是一个重要的调节机制.
- 这些发现突显了内细胞形成的可塑性,以应对血管系统内的生物物理力量.
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