纤维素还原端和初级基组的修改对纤维素-纤维素酶相互作用和纤维素水解的影响
Rajeev Kumar1,2, Samarthya Bhagia1,3, Ashutosh Mittal4
1Center for Environmental Research and Technology (CE-CERT), Bourns College of Engineering, University of California Riverside, Riverside, California, USA.
Biotechnology and bioengineering
|June 10, 2024
概括
修改纤维素减少端并没有显著影响纤维素的可访问性或水解,与普遍认为的相反. 然而,初级基组的氧化确实降低了纤维素的转化率.
科学领域:
- 生物质转化和生物能源
- 生物催化和酶工程 生物催化和酶工程
- 纤维素化学 纤维素化学
背景情况:
- 假设纤维素的减少端有助于纤维素的回收,阻碍酶转化.
- 这些还原端在纤维素可访问性和水解中的具体作用尚不清楚.
研究的目的:
- 调查修改纤维素减少端对纤维素结构,可访问性和酶化水解的影响.
- 为了澄清降解端与其它纤维素修饰在回性中所扮演的角色.
主要方法:
- 来自各种来源的不溶性纤维素被减少剂 (酸) 和氧化剂 (酸-酸,双酸) 处理,以修改减少端.
- 使用X射线衍光度测量的结构分析评估了纤维素结晶性的变化.
- 使用完整和纯化的纤维素成分评估了纤维素的可访问性和水解率.
主要成果:
- 化和双酸的处理分别修改了90%以上和不同程度的减少端,而没有改变纤维素的结晶性.
- 通过玻利或双酸修改降解端对纤维素可访问性和水解率的影响微不足道.
- 酸酸氧化显著降低了纤维素的转化,但这归因于初级基组氧化,而不是减少末端修饰.
结论:
- 在测试条件下,纤维素减少端的修改对纤维素的可访问性和酶化水解的影响最小.
- 主要基组的修改,而不是减少终端修改,通过影响可访问性和酶吸收,显著影响纤维素转化.
- 这些发现挑战了将削减末端作为纤维素复原的主要因素的普遍观念.
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