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通过综合性血清药理学,转录学和网络药理学来探索 Sendeng-4 抗风湿性关节炎的机制

Chenyue Pei¹, Jiuwang Yu¹, Guanglong Wang²

¹Hohhot Hospital of Traditional Chinese Medicine and Mongolian Medicine, Hohhot, Inner Mongolia, P. R. China.

Biomedical chromatography : BMC

|June 10, 2024

概括

蒙古医学 Sendeng-4 (SD-4) 通过调节PI3K-Akt信号通路,有效治疗类风湿性关节炎 (RA). 这项研究确定了生物活性成分,并证实了SD-4的存在.

科学领域:

* 药理学药理学药理学
* 传统中国医疗
* 风湿病学风湿病学

背景情况:

蒙古药Sendeng-4 (SD-4) 显示了对类风湿性关节炎 (RA) 的临床疗效.
在RA治疗中,SD-4的生物活性成分和潜在机制尚不清楚.

研究的目的:

探索SD-4的生物活性化学成分.
阐明SD-4在治疗类风湿性关节炎 (RA) 的机制.
在动物模型中验证SD-4的抗RA作用.

主要方法:

已建立的高性能液体染色学-并联质谱法 (HPLC-MS/MS) 用于组件量化.
使用超高性能LC-Q-Exactive-MS/MS来识别血清吸收的植物化学物质.
雇佣了网络药理学,转录学,分子对接,以及用于机制验证的老鼠RA模型.

主要成果:

在血清样本中确定了37种化合物;SD-4中的15种主要成分显示出良好的线性.
网络药理学和转录学确定了酸丁3-激酶 (PI3K) -蛋白激酶B (Akt) 信号通路作为关键.
在大鼠模型中,SD-4治疗显著降低了RA症状,炎症和PI3K-Akt通路激活.

结论:

SD-4通过调节PI3K-Akt信号通路来缓解类风湿性关节炎 (RA).
这项研究为SD-4在RA的临床使用提供了理论基础.
提供了在传统医学中识别生物活性物质和机制的策略.

关键词:

这就是PI3KAktAkt.SD‐4 SD‐4 SD‐4 SD‐4 SD‐4 SD‐4 SD‐4 SD‐4 SD‐4 SD‐4 SD‐4 类风湿性关节炎类风湿性关节炎血清中的植物化学.转录组学转录组学是指转录组学.

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