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相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Label-Free Immunoprecipitation Mass Spectrometry Workflow for Large-scale Nuclear Interactome Profiling
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使用多功能线性离子陷开发有针对性的蛋白质组学试验工作流程.

Ariana E Shannon, Rachael N Teodorescu, Nojoon Soon

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    此摘要是机器生成的。

    混合四极线性离子陷仪器使全球数据独立采集 (DIA) 测量无需高质量精度的成本效益有针对性的蛋白质组学成为可能. 这种方法允许快速测试开发和一致的量化,即使在有限的样本输入.

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    科学领域:

    • 质谱测量质量谱测量
    • 蛋白质组学是指蛋白质组学.
    • 分析化学 分析化学

    背景情况:

    • 单细胞蛋白质组学需要高质量精度仪器,限制了可访问性.
    • 三重四极仅限于针对性蛋白质组学.
    • 线性离子陷 (LITs) 为目标和全球蛋白质组学提供了具有成本效益的替代方案.

    研究的目的:

    • 用混合四极-LIT仪器演示开发向蛋白质组学试验的工作流程.
    • 通过全球数据独立采集 (DIA) 实现有针对性的蛋白质组学,而无需高质量精度.
    • 为了评估这个工作流的性能,量化线性和一致性.

    主要方法:

    • 使用混合四极线性离子陷 (LIT) 质谱仪.
    • 基于气相分数的数据独立采集 (DIA) 用于目标库生成.
    • 使用EncyclopeDIA软件进行并行反应监测 (PRM),测试调度和定量.

    主要成果:

    • 在输入材料的三个数量级上实现了一致的量化.
    • 证明了的定量线性,直到25倍稀释,只有1 ng蛋白质组.
    • 流细胞计免疫细胞种群和基于LIT的蛋白质组免疫标记物之间在1 ng总蛋白质时显示一致性.

    结论:

    • 混合四极LIT仪器为向蛋白质组学提供了一个经济的解决方案.
    • 这种工作流使质谱学在各种实验室环境中实现了民主化.
    • 允许敏感和定量蛋白质组学,而不需要稳定的同位素标记标准.