VapB抗毒素的酸化影响分子间相互作用,以调节Mycobacterium结核病中的VapC毒素活性
Basanti Malakar1,2, Valdir Barth1,2, Julia Puffal3
1Division of Infectious Diseases, Boston Children's Hospital, Boston, MA, USA.
bioRxiv : the preprint server for biology
|June 10, 2024
概括
在Mycobacterium tuberculosis中VapB抗毒素的酸化调节了VapC毒素的活性. 这种酸化控制了VapB-VapC相互作用和基因表达,影响了细菌毒性.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 像VapBC这样的毒素-抗毒素模块在细菌病原体如Mycobacterium tuberculosis中至关重要.
- VapB抗毒素通常会抑制VapC毒素并抑制其自身的基因表达.
研究的目的:
- 研究VapB蛋白酸化在调节VapB-VapC相互作用和M.结核病中VapC毒性的作用.
- 探索酸化如何影响VapB与其同类VapC毒素和促进DNA的结合.
主要方法:
- 用局部导向的突变发生法来创建相仿和可塑性VapB变体.
- VapB-VapC相互作用通过共免疫沉或类似的测定来评估.
- 分析了VapB与DNA促进子区域的结合.
- 对表达不同VapB变异的M.结核菌株进行了毒性测试.
主要成果:
- 模仿性VapB替代物减少了与VapC毒素的相互作用,并损害了VapB与促进DNA的结合.
- 基替代剂没有显著改变VapB-VapC结合或DNA结合.
- 随着相仿VapB突变,观察到VapC活性和毒性增加.
结论:
- VapB酸化是一种新型的调节机制,可以控制M.结核病中的VapC毒素活性.
- 这种酸化会影响VapB-VapC相互作用和转录抑制,从而调节细菌毒性.
- VapB酸化可以作为对内部和外部细胞信号的反应.
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