在棒光受体发育过程中,CTCF调节了全球染色体的可访问性和转录
bioRxiv : the preprint server for biology
|June 10, 2024
概括
建筑蛋白CTCF在小鼠棒发育过程中调节了全球色素可访问性和基因表达. 削弱CTCF显著改变了20%以上的转录组和40%的基因组可访问性.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 染色体架构影响基因转录,但其在全球调节中的作用仍在争论中.
- 以前的研究表明,在结构性蛋白质耗尽后,基因表达变化有限,可能是由于细胞异质性.
研究的目的:
- 研究建筑蛋白CTCF在全球染色体可访问性和基因表达调节中的作用.
- 为了解决在以前的研究中观察到的染色体组织变化和基因表达改变之间的差异.
主要方法:
- 进行了对分类后转移性小鼠棒进行多组分析,这些小鼠棒表现出同步发育.
- 使用CRISPR干扰 (CRISPRi) 介导的CTCF枯竭来评估其功能影响.
主要成果:
- CTCF枯竭失调了大约20%的转录组 (>3,000个基因) 和41%的基因组可访问性 (>26,000个位点).
- 受影响的区域富含欧克罗马丁和CTCF占用量,这表明活性位点的直接调节.
- 结合CTCF主要促进了促进子的染色质可访问性,经常抑制基因表达,并丰富了抑制因子.
结论:
- 在鼠标棒发育过程中,CTCF是全球色素可访问性和转录的关键调节者.
- 细胞异质性可以掩盖转录变化的全部程度;细胞类型特定的多组学分析至关重要.
- 在调节活性位点方面,CTCF的作用包括促进可访问性,同时可能抑制特定位点的表达.
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