阴道微生物改变了表皮转录组,并诱导了表观基因组的修饰,为易受不良生殖结果的机制提供了洞察力
Michal Elovitz1, Lauren Anton2, Ana Cristancho3
1Icahn School of Medicine at Mount Sinai.
Research square
|June 10, 2024
概括
阴道菌 (Gardnerella vaginalis) 触发女性生殖道中的免疫反应和细胞死亡. 乳杆菌crispatus超水生物改变宫细胞表观遗传学,建议基于微生物组的生殖健康疗法.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 宫和阴道微生物组的组成会影响女性的生殖健康.
- 乳酸菌的优势是最佳的,而无氧生物的丰富性,如Gardnerella vaginalis,与不良结果有关.
- 在下生殖道中宿主-微生物相互作用的机制尚未完全理解.
研究的目的:
- 为了研究宫性阴道上皮的转录反应对阴道细菌和它们的超水生物.
- 阐明受宿主微生物相互作用影响的下生殖道中的分子通路.
主要方法:
- 宫上皮细胞的体外表征.
- 用RNA测序来分析转录的变化.
- ATAC测序用于评估表观基因组景观修改.
主要成果:
- 加德内瑞拉阴道菌上调了与先天性免疫反应和炎症酶通路 (NLRP3,caspase-1,IL-1β) 相关的基因,诱导细胞死亡.
- 活生生的 Lactobacillus crispatus 对上皮细胞的转录学影响很小.
- 乳杆菌crispatus超引发了表观基因组变化,包括宫细胞中的染色质可访问性降低.
结论:
- 阴道菌激活特定的宿主免疫路径,包括炎细胞激活和细胞死亡.
- 乳杆菌crispatus影响子宫细胞的表观基因组景观,表明它在维持表皮细胞平衡中发挥了作用.
- 研究结果表明,针对阴道微生物群的潜在治疗策略可以改善生殖健康.
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