相关实验视频

Updated: Jun 24, 2025

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

在代谢提取物度过程中挥发性代谢物的损失

Nataliya A Osik¹, Nikita N Lukzen^1,2, Vadim V Yanshole^1,2

¹International Tomography Center Siberian Branch of Russian Academy of Sciences, Institutskaya str. 3a, Novosibirsk 630090, Russia.

|June 10, 2024

概括

在样本干燥过程中挥发性代谢物损失对于一些化合物来说是不可避免的. 然而,在代谢分析中保留分离的挥发性酸可以通过使用缓冲盐控制pH值或调整干燥来改善.

科学领域:

分析化学分析化学
代谢学代谢学代谢学
生物化学生物化学

背景情况:

预分析样本处理可能导致挥发性代谢物损失.
了解蒸发和保留机制对于准确的代谢分析至关重要.

研究的目的:

研究提取物溶液干燥过程中挥发性代谢物蒸发和保留的机制.
确定影响代谢样本中挥发性化合物的损失或保留的因素.

主要方法:

关于挥发性物质蒸发和保留的实验研究.
在溶剂蒸发和干燥过程中对代谢物行为的理论分析.
研究缓冲盐和pH对挥发性化合物稳定性的作用.

主要成果:

在溶剂蒸发过程中,不可分离的挥发性代谢物 (如乙,乙醇) 丢失.
分离的挥发性酸的保留取决于缓冲盐保持中性pH值.
在晚期干燥阶段的缓冲盐沉可以捕获代谢物,但也增加了挥发.

结论:

在样本准备过程中挥发性代谢物损失是代谢学中的一个重大挑战.
优化缓冲盐度和干燥条件可以改善挥发性有机酸的回收.
精心处理分析前的样本对于可靠的代谢学数据至关重要.

更多相关视频

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

相关概念视频

Volatilization

Volatilization

Volatilization gravimetry is an analytical technique that measures the mass lost due to the volatilization of the substance. This technique is used to estimate the amount of volatile material in a sample. To perform this method, heat a known amount of the sample to a high temperature in a crucible or other suitable vessel. The volatile substance in the sample evaporates, and the vapor is completely expelled from the crucible either by heating the sample or bubbling a stream of inert gas through...

Distillation: Vapor–Liquid Equilibria

Distillation: Vapor–Liquid Equilibria

Distillation is a separation technique that takes advantage of the boiling point properties of disparate elements in a mixture. To perform distillation, we begin by heating a miscible mixture of two liquids with a significant difference in boiling points (at least 20°C). As the solution heats up and reaches the bubble point of the more volatile component, some molecules of the more volatile component transition into the gas phase and travel upward into the condenser, which is a glass tube...