富含的水通过通过sirtuin1/核因子-erythroid-2-相关因子2/血氧酶-1信号通路减轻氧化应激减轻便秘
Kai-Di Chen1,2, Kui-Ling Wang1,2, Chen Chen1,2
1Jiangsu Province Hospital of Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China.
富含的水 (HRW) 通过减少氧化应激和改善肠道健康,有效治疗便秘. 这种疗法调节肠道微生物群,并向SIRT1/Nrf2/HO-1通路,以提高疗效.
科学领域:
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
- 微生物组研究 微生物组研究
背景情况:
- 便秘是一种常见的功能性胃肠道疾病,严重影响患者的生活质量和医疗费用.
- 氧化应激是结肠不运动和便秘的关键因素.
- 疗是一种有前途的干预措施,用于便秘管理.
研究的目的:
- 研究富含水 (HRW) 在缓解便秘方面的有效性.
- 阐明HRW对便秘的治疗效果的潜在机制.
主要方法:
- 在使用洛佩拉米德的斯普拉格-道利大鼠中引起便秘,随后给予HRW.
- 分析包括便参数,肠道微生物群测序,血清代谢量和结肠组织评估 (组织学,关键通路的蛋白质/基因表达).
- 在一个救援实验中使用了一种SIRT1抑制剂,细胞模型评估了代谢物对氧化应激的影响.
主要成果:
- 人权观察改善了便秘症状,包括增加便重量,便含水量和肠道过渡.
- HRW调节了肠道微生物群,通过SIRT1/Nrf2/HO-1通路纠正了血清代谢异常,并减少了氧化应激.
- 特定的代谢物β-氨酸和创伤性酸被确定为可能上调SIRT1并减少氧化应激.
结论:
- 通过SIRT1/Nrf2/HO-1通路减少肠道氧化应激,HRW减轻了便秘.
- 人权观察还对肠道微生物群和血清代谢物产生积极影响.
- 乙-氨酸和创伤性酸通过上调SIRT1和减轻氧化应激,显示出作为治疗剂的潜力.
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