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在膀癌中探索与solazonine相关的microRNA-mRNA调节网络

Kun Fang^1,2, Da-Lang Fang³, Hui Yu⁴

¹Nanjing University of Chinese Medicine, Nanjing, China.

Translational andrology and urology

|June 10, 2024

概括

这项研究揭示了索拉索宁如何通过识别关键的微RNA (miRNA) 和信使RNA (mRNA) 来抑制膀癌 (BC). 一个索拉索宁-miRNA-mRNA调节网络强调miR-450b-5p对于索拉索宁的抗癌作用至关重要.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

索拉索宁显示出对膀癌 (BC) 的抑制作用.
索拉索宁在BC中的抗瘤活性背后的精确分子机制尚未完全理解.
研究微RNA (miRNA) 中介调节对于阐明这些机制至关重要.

研究的目的:

为了探索miRNA介导调节和solasonine在膀癌中的抗瘤活性之间的关联.
构建一个全面的solasonine-miRNA-mRNA调节网络.
为了确定关键的miRNAs和信使RNAs (mRNAs) 参与solazonine的抗BC作用.

主要方法:

微RNA (miRNA) 测序被用来识别用solazonine治疗的BC细胞中差异表达的miRNA (DE-miRNA).
对激活和抑制的DE-miRNA进行了功能丰富分析.
生物信息学方法,包括生存分析,表达评估和蛋白质-蛋白质相互作用分析,用于识别枢纽DE-miRNA和核心下游基因.

主要成果:

总共有46个索拉索宁介导的DE-miRNA (27个激活,19个抑制) 被确定,与瘤相关的生物功能有关.
确定了9个具有预后价值和BC中的差异表达的枢纽DE-miRNA.
构建了一个单氨酸-miRNA-mRNA调节网络,涉及30个核心下游基因,其中hsa-miR-450b-5p被确定为关键调节器.

结论:

在膀癌中成功构建了一种涉及索拉索宁,miRNA和mRNA的新型调节网络.
这个网络为推动索拉索宁在BC的抗癌疗效的分子机制提供了重要的见解.
hsa-miR-450b-5p在索拉松因介导的抗膀癌作用中发挥着关键作用.

关键词:

膀癌 (BC) 是一种癌症.微RNA测序 (miRNA-seq) 是指微RNA的测序方式.微型RNAs (miRNAs) 是一种微型RNA.索拉索尼尼 (Solasonine) 是一种可靠的药物.

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