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可调的液态透镜用于三光子激发显微镜

Samuel D Gilinsky¹, Diane N Jung², Greg L Futia³

¹Department of Electrical, Computer, and Energy Engineering, University of Colorado Boulder, Boulder, Colorado 80309, USA.

Biomedical optics express

|June 10, 2024

概括

研究人员使用室温离子液体 (RTIL) 和1--1-环烯 (PCH) 开发了一种新的电湿透镜. 这种可调节镜头非常适合用于可调节焦点的3光子显微镜应用.

科学领域:

光学和光子学在光学和光子学.
生物医学成像技术生物医学成像技术
材料科学材料科学材料科学

背景情况:

电湿透镜提供可调节的光学特性.
3光子显微镜需要先进的光学组件来进行深层组织成像.
离子液体和特定的非极性液体具有独特的光学特征.

研究的目的:

为了展示一种新的电湿液体组合,用于可调焦的3光子显微镜.
描述液体组合在1300 nm的光学特性.
在3光子成像系统中验证电湿透镜的性能.

主要方法:

使用室温离子液体 (RTIL) 和1--1-环烯 (PCH) 进行电湿透镜制造.
在1300nm测量了光学传输和折射率对比度.
应用电压来调整镜头的接触角度和焦点特性.
将电湿透镜集成到一个用于成像生物样本的3光子显微镜系统中.

主要成果:

无论是RTIL还是PCH,都显示了超过90%的传输在1300nm超过1.1mm.
实现了0.123.3的折射对比度指数.
经过证明,可调节的接触角度从133°到48°,电压为0-60V.
通过电透镜成功成像了小鼠大脑切片,显示了与机械扫描目标相似的结果.

结论:

RTIL和PCH液体组合适用于可调焦的3光子显微镜.
电透镜为轴向成像提供了机械扫描的可行替代方案.
这项技术增强了先进的生物成像技术的能力.

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