理论和实验确定OCT系统的对焦功能,以准确计算样品的光学特性
Gijs Buist1, Maddalena Debiasi1, Arjen Amelink1,2
1LaserLaB, Department of Physics and Astronomy, Vrije Universiteit, Amsterdam, The Netherlands.
Biomedical optics express
|June 10, 2024
概括
这项研究模拟了生物组织光连贯断层扫描 (OCT) 中的共聚焦功能. 准确的对焦功能建模对于确定组织衰减系数至关重要,有助于诊断疾病.
科学领域:
- 生物医学光学 生物医学光学
- 医疗成像医学成像
- 生物光子学 生物光子学
背景情况:
- 生物组织的减弱系数表明疾病的进展.
- 光学连贯断层扫描 (OCT) 图像使用反射光的组织结构.
- 准确的OCT强度分析需要对Lambert-Beer衰变的对焦功能进行表征.
研究的目的:
- 展示使用高斯束照明的散射介质中对聚焦函数的模型.
- 将模型与镜子反射进行比较,并与OCT实验数据进行验证.
- 使用开发的模型从散射样本中提取衰减系数.
主要方法:
- 模拟使用Huygens-Fresnel原理用于高斯束照明和单模光纤检测的对焦函数.
- 将该模型的雷利利射程与镜子反射进行比较.
- 在镜子,Spectralon和Intralipid稀释剂上进行OCT焦点系列测量.
- 提取衰减系数使用单散射模型,结合着对焦函数.
主要成果:
- 开发的模型显示相同的对焦功能 (以雷利范围为特征) 散射介质和镜像反射,与之前的文献相反.
- 海洋和海上国家/地区的测量证实了模型,并揭示了高度分散的样本中的深度依赖的共焦功能.
- 从内部脂质稀释中提取的减弱系数与已知的弱散射样本 (μ < 2 mm−1) 的已知值非常一致.
结论:
- 拟议的模型准确地描述了OCT的散射介质中的对焦函数.
- 这项研究强调了在高度分散的组织中对焦点功能的深度依赖性.
- 使用OCT精确确定组织衰减系数是可行的,这对疾病诊断有意义.
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