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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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在多光子显微镜中用于NADH光检测的高度选择性的光学过器.

R B Freitas1, M J L F Rodrigues2, S Pimenta1,3

  • 1CMEMS-UMinho, University of Minho, 4800-058, Guimarães, Portugal.

Biomedical optics express
|June 10, 2024
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概括

一个新的光学波器精确地检测尼古丁胺氨酸二核酸 (NADH) 光,使用双光子激发光 (TPEF) 来改善结直肠癌 (CRC) 检测. 这项技术有助于开发用于早期诊断的非侵入性光学活检.

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科学领域:

  • 生物医学光学 生物医学光学
  • 材料科学 材料科学 材料科学
  • 癌症的诊断 癌症的诊断

背景情况:

  • 结肠直肠癌 (CRC) 是一个重大的全球健康挑战.
  • 早期发现CRC对于有效治疗和改善患者治疗结果至关重要.
  • 目前的诊断方法可能是侵入性的,缺乏实时分子信息.

研究的目的:

  • 开发和描述一种新型的光学波器,用于精确检测尼古丁胺氨酸二核酸 (NADH) 光.
  • 允许使用双光子激发光 (TPEF) 进行增强的结直肠组织分析.
  • 推进多光子显微镜在结肠镜中进行非侵入性光学活检的整合.

主要方法:

  • 在Fabry-Perot结构中使用二氧化和二氧化薄膜制造光学过器.
  • 过器的光学特性,包括峰值透射率和半最大的全宽度的表征.
  • 两光子激发光 (TPEF) 测量使用与NADH液体幻影量身定制的设置.

主要成果:

  • 开发的过器在483 nm时的峰值传导率约为95%.
  • 过器显示了狭窄的带宽,在12nm的最大半幅最大的全宽带宽.
  • 过器有效地隔离了NADH光,最大限度地减少了生物样本中其他潜在光体的干扰.

结论:

  • 开发的光学波器是高效的选择性NADH光检测通过TPEF.
  • 这项技术代表了将光学活检整合到结肠镜中用于结肠直肠组织分析的重大进步.
  • 进一步的实验设置改进是必要的,以改善组织分化和增强CRC诊断能力.