甜玉米植物糖原 dendrimers 作为干燥状态蛋白质储存的冷保护剂
Junha Park1, Renjie Liu1, Alexander S Kim2
1J. Crayton Pruitt Family Department of Biomedical Engineering, Wertheim College of Engineering, University of Florida, Gainesville, Florida, USA.
Journal of biomedical materials research. Part A
|June 10, 2024
概括
甜玉米植物甘油素 (PG) 作为蛋白质生物治疗的新型冷保护剂和蛋糕形成剂. 这些葡萄糖树状体减少了冷链储存需求,通过使稳定的干燥蛋白质配方.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
- 药物运输 药物运输 药物运输
背景情况:
- 蛋白质生物疗法需要冷链储存以保持稳定.
- 化为冷链储存提供了替代方案,但需要优化的辅助剂.
- 助剂必须提供冷保护,冷保护和脱水蛋白的蛋糕形成.
研究的目的:
- 研究甜玉米植物甘油素 (PG) 作为蛋白质冷解的双重功能辅助剂.
- 为了确定具有最佳冷保护和蛋糕形成特性的特定PG变体.
- 建立PG树突体作为干式蛋白质配方的新类助剂.
主要方法:
- 从玉米中提取和描述16种植物糖原变体 (PG1-16).
- 评估PG的体稳定性,蛋白质污染物水平和细胞相容性.
- 评估PG通过多个冷化周期保持蛋白质活性的能力.
- 蛋白质-PG混合物的玻璃过渡温度 (Tg') 和蛋糕形态的确定.
主要成果:
- 植物甘油素 (PG) 树突体表现出热保护和蛋糕形成的能力.
- 特定的PG变体 (PG1,2,9,13,15,16) 在溶解后保持了蛋白质活性.
- 由于其狭窄的分散性,稳定性,低污染物和细胞相容性,PG13成为主要候选者.
- 最佳的冷保护和PG13的蛋糕形成依赖于密度,需要至少5mg/mL.
结论:
- 植物糖原树枝状体代表了一种用于蛋白质冷化的新型辅助剂类.
- 蛋白质原料为传统辅助剂提供了一个有希望的替代品,简化了蛋白质配方.
- 这些发现有助于开发稳定,可在室温储存的蛋白质生物疗法.
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