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相关概念视频

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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相关实验视频

Updated: Jun 24, 2025

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble
06:50

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

Published on: June 5, 2021

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通过Web接口使用EMBL-EBI服务,并通过Web服务使用程序.

Fábio Madeira1, Nandana Madhusoodanan1, Joonheung Lee1

  • 1European Molecular Biology Laboratory-European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom.

Current protocols
|June 10, 2024
PubMed
概括
此摘要是机器生成的。

欧洲生物信息学研究所 (EMBL-EBI) 提供网络服务和Docker容器,以访问生物信息学数据库和工具. 研究人员可以使用Perl,Python和Java的客户端将这些资源集成到工作流中.

关键词:
休息时间 休息时间肥 肥 是一种肥.生物信息学资源 生物信息学资源生物信息学工具 生物信息学工具程序性访问是指程序性访问.网络服务 网络服务工作流程的工作流程.

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Last Updated: Jun 24, 2025

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The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

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Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web
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Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

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科学领域:

  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学
  • 数据科学数据科学数据科学

背景情况:

  • 欧洲生物信息学研究所 (EMBL-EBI) 提供重要的生物信息学数据库和分析工具.
  • 对这些资源的程序性访问对于将它们整合到分析工作流和管道中至关重要.
  • 网络服务 (REST和SOAP) 和容器化 (Docker) 提供灵活的集成选项.

研究的目的:

  • 描述在程序上访问EMBL-EBI资源的方法和选择.
  • 引导研究人员和生物信息学家利用网络服务和Docker容器进行生物信息学分析.
  • 为了促进EMBL-EBI工具在定制分析管道中的集成.

主要方法:

  • 在Perl,Python和Java中使用RESTful网络服务客户端进行数据检索和分析.
  • 使用Docker容器,将EMBL-EBI资源无集成到分析工作流中.
  • 展示数据检索 (Dbfetch),序列相似性搜索 (FASTA,BLAST+,HMMER3),蛋白质功能分析 (InterProScan 5) 和多个序列对齐 (Clustal Omega) 的特定协议.

主要成果:

  • 通过各种客户端和协议,EMBL-EBI资源的成功程序访问得到了证明.
  • 将核心生物信息学工具集成到分析管道中,可以使用所提供的方法实现.
  • 这篇文章作为一本实用指南,为各种生物信息任务提供了详细的协议.

结论:

  • 通过Web服务和Docker,EMBL-EBI资源可以轻松访问和集成到计算工作流中.
  • 研究人员可以利用这些工具来提高他们的生物信息学分析和数据处理能力.
  • 描述的方法使生物信息学家能够构建可靠和可重复的分析管道.