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相关概念视频

Histone Variants at the Centromere02:30

Histone Variants at the Centromere

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Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
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Centrosome Duplication02:25

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The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
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Centrioles and Centrosomes01:13

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Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
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Separation of Sister Chromatids02:17

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At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
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The Spindle Assembly Checkpoint02:19

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The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
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Attachment of Sister Chromatids02:57

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As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
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相关实验视频

Updated: Jun 24, 2025

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
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Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

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Cep57通过多价值相互作用来调节人类的中心体.

Hung-Wei Yeh1, Po-Pang Chen1, Tzu-Chen Yeh1

  • 1Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu 30013, Taiwan.

Proceedings of the National Academy of Sciences of the United States of America
|June 10, 2024
PubMed
概括
此摘要是机器生成的。

人类Cep57蛋白通过组织细胞中心体来控制细胞分裂. 它的相分离,由Cep63调节,对于微管形成和中心体完整性至关重要,其中的突变与积体综合征有关.

关键词:
在Cep57和Cep57之间在Cep63的Cep63.中心体的中心体是什么液体液体相分离器 液体相分离器微管细胞核化的过程

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Last Updated: Jun 24, 2025

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Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
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科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 人类Cep57是一种围心矩阵支架蛋白质,对于中心重复和中心成熟至关重要.
  • 在Cep57中发生的突变与马赛克多样化积体 (MVA) 综合征有关.
  • 在相间期间,Cep57与Cep63和Cep152相互作用,但它们的分子相互作用尚未完全理解.

研究的目的:

  • 阐明Cep57在中心体组织中的功能背后的分子机制.
  • 调查Cep57在相分离和微管核化能力中的不同域的作用.
  • 了解Cep63如何调节Cep57的活动以及MVA突变如何影响Cep57的功能.

主要方法:

  • 在Cep57液体液相分离 (LLPS) 的体外表征使用纯化域.
  • 在体外微管核化试验中使用Cep57凝聚剂.
  • 细胞实验涉及Cep57枯竭,突变物过度表达,以及对心体结构和功能的分析.

主要成果:

  • Cep57经历了由其NTD,CTD和LMN域驱动的LLPS.
  • 在体外,Cep57凝结物通过LMN动机介导的氨酸度促进微管核形成.
  • 这种LMN基因对细胞中中枢细胞微管星的形成至关重要,而Cep63限制了Cep57的组装和活动.
  • 与MVA相关的突变和竞争结构的过度表达导致过度的中枢细胞组重复,而Cep57的枯竭导致PCM的混乱.

结论:

  • Cep57接受LLPS的能力对于其作为中心体支架的功能至关重要.
  • 由Cep57调解的多价值相互作用,特别是其LMN动机,对于准确的微管核和中心体结构完整性至关重要.
  • 对Cep57相分离和相互作用的调节失调有助于阳性积分和中心体重复缺陷.