从石竹 (Lentinula edodes) 果实体中提取基-葡萄糖复合物,使用天然的深度浸泡溶剂及其益生菌潜力
Sayari Majumdar1, Pradeep Singh Negi1
1Fruit and Vegetables Technology Department, CSIR-Central Food Technological Research Institute, Mysuru 570 020, India.
International journal of biological macromolecules
|June 10, 2024
概括
来自石竹的基-葡萄糖复合物 (CGC) 显示出强大的益生菌潜力. 通过使用天然的深溶性溶剂和发酵进行优化提取,可以获得具有抗氧化和有益肠道健康特性的稳定化合物.
科学领域:
- 生物化学 生物化学
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
背景情况:
- 基-葡萄糖复合物 (CGC) 已因其生理上的好处而得到认可.
- 需要新的提取方法来优化CGC产量和性能.
- 衍生的多糖的 prebiot 的潜力需要进一步的研究.
研究的目的:
- 从石竹果实体中提取和特征化基-葡萄糖复合物 (CGC).
- 评估提取的CGC的物理化学性质和益生菌活性.
- 评估CGC在营养药应用中的稳定性和适用性.
主要方法:
- 采用天然的深溶性溶剂 (NADES),超声波和用益生菌微生物进行浸泡发酵来提取CGC.
- 使用rheological分析,FTIR,1D和2DNMR和XRD进行表征.
- 对抗氧化剂潜力,益生菌活性和短链脂肪酸 (SCFA) 生产的评估.
- 在体外消化模拟以评估在酸性条件下的稳定性.
主要成果:
- 经过优化提取,通过使用Lactiplantibacillus plantarum进行非超声波发酵,获得了最大的多糖化物 (27.86%).
- NADES (尿素:葡萄糖) 组合显示出优越的抗氧化能力.
- CGC表现出剪切稀释的质行为,特有的光谱图案 (FTIR,NMR) 和晶体结构 (XRD).
- 观察到增强的激素清除活性,显著的益生菌潜力 (118-134%) 和SCFA释放 (9.99 mM).
- 在模拟的胃条件下,CGC显示稳定性.
结论:
- 优化的提取方法可以提高CGC产量和抗氧化特性.
- 具有特征的CGC具有有利的物理化学和结构属性,可用于前生物的应用.
- 在酸性环境中CGC的稳定性支持其在含营养药物丰富的食品中使用.
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