试虫中的内生共生:细菌的分裂取决于微管的活力
Júlia Ribeiro Moraes1, Azuil Barrinha1, Luan Santana Gonçalves de Lima1
1Laboratório de Ultraestrutura Celular Hertha Meyer, Centro de Pesquisa em Medicina de Precisão (CPMP), Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro-UFRJ, 21491-590, Rio de Janeiro, RJ, Brazil.
Experimental cell research
|June 10, 2024
概括
微管子的活力影响了三生虫中的共生细菌分裂. 在Angomonas deanei中抑制基因组脱乙酶6 (HDAC6) 引起了共生体线索,证明了微管子重塑影响 prokaryote 细胞分裂.
科学领域:
- 细胞生物学 细胞生物学
- 微生物学 微生物学
- 寄生虫学的寄生虫学
背景情况:
- 微管是真核细胞骨的重要组成部分,参与细胞分裂和运动.
- 在三体动物中,微管对于细胞组织和细胞内运输至关重要.
- 一些试虫中的共生细菌与宿主细胞结构协调分裂,这一过程已知依赖于微管.
研究的目的:
- 为了研究微管的活力和重塑是否会影响试管动物中的共生细菌的分裂.
- 探索基因组脱乙酶6 (HDAC6) 在调节微管体动态中的作用及其对共生生物分裂的影响.
主要方法:
- 用Trichostatin A (TSA) 治疗Angomonas deanei,这是一种脱乙酶抑制剂.
- 使用CRISPR-Cas9技术生成HDAC6缺乏突变细胞.
- 原生动物及其共生细菌的微观和超结构分析.
主要成果:
- TSA治疗和HDAC6缺乏导致原生动物增殖的减少.
- 在受治疗和突变细胞中观察到增强的素乙化和显著的形态/超结构变化.
- 在两种实验条件下都发生了共生体线,表明细菌分裂受损.
结论:
- 微管的活力和重塑对于试生菌中共生细菌的适当分裂至关重要.
- HDAC6在调节影响 prokaryote 细胞分裂的微管子动力学方面发挥着作用.
- 这些发现突显了宿主微管细胞骨架和共生细胞循环调节之间的复杂相互作用.
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