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暴露于DBP会通过降低FOXO3a表达的调节影响卵细胞通过卵巢粒状细胞的细胞外囊衍生的miR-116-5p受精

Hongmei Liao¹, Wenqu Tian¹, Wen Yao¹

¹Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.

Reproductive sciences (Thousand Oaks, Calif.)

|June 10, 2024

概括

妇女暴露于单乙 (MBP) 与卵泡液中细胞外囊泡微RNA (EV-miRNA) 发生变化有关,可能会损害卵细胞发育和受精. MBP特别增加了miR-116-5p,它抑制了FOXO3a,影响了受精.

科学领域:

生殖毒理学生殖毒理学
环境健康环境健康
分子生物学分子生物学

背景情况:

单乙烯甲酸 (MBP) 是乙烯甲酸代谢物,在中国女性中很普遍.
卵泡液中的细胞外囊泡 (EVs) 和它们的微RNA (EV-miRNAs) 对于女性的繁殖至关重要.
暴露于甲酸盐是对生殖健康的日益关注的问题.

研究的目的:

调查DBP暴露,毛囊液中的EV-miRNA配置文件和卵细胞发育受损之间的关联.
探索DBP暴露影响女性生育能力的潜在机制.
为了确定特定的EV-miRNAs在毛囊液中因DBP暴露而改变.

主要方法:

在高 (A组) 和低 (B组) 的DBP暴露的女性之间,在FF-EVs中的miRNA表达的比较,具有不同的受精状态.
目标基因的预测和差异表达miRNAs的功能分析.
在体外暴露KGN细胞对MBP以验证miRNA表达变化并研究机制.

主要成果:

在高和低DBP暴露组之间,一些miRNA在FF-EV中被差异地表达.
在实验室中,MBP暴露显著增加了EVs中的miR-116-5p水平.
确定了miR-116-5p作为一个关键的调解剂,抑制FOXO3a并影响卵细胞受精.

结论:

毛囊液中的EV-miRNA可能充当MBP对卵细胞毒性的调解者.
暴露于MBP可以通过改变EV-miRNA配置文件来破坏女性生殖健康.
miR-116-5p是DBP诱导的生殖功能障碍的潜在生物标志物和治疗标.

关键词:

细胞外囊泡细胞外囊泡毛囊液是什么? 毛囊液是什么?这是一个微型RNA.卵细胞是卵细胞中的一个.甲酸是一种甲酸.

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