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系统地选择合适的参考基因,用于对Lutjanus erythropterus的基因表达进行定量实时PCR规范化研究

Lujun Chen1, Qiulu Liang1, Zhuoxin Lai1

  • 1Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang, 524025, China.

Scientific reports
|June 10, 2024
PubMed
概括

这项研究确定了最好的参考基因,用于精确的基因表达分析红海 (Lutjanus erythropterus). 对于不同的实验条件,建议使用RAB10,PFDN2,NDUFS7和MRPL17.

关键词:
卢塔努斯 (Lutjanus erythropterus) 是一种红色类动物.参考基因是一种基因.转录组 转录组就是一个转录组.qRT-PCR 是一个很好的方法.

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...