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通过流细胞计检测CLCF1蛋白表达的检测

Véronique Laplante¹, Marine Rousseau², Félix Lombard-Vadnais³

¹Département de pharmacologie et physiologie, Université de Montréal, Montréal, QC, H3T 1J4, Canada.

Scientific reports

|June 10, 2024

概括

研究人员开发了一种流细胞测量方法,以检测免疫细胞中的心心类细胞因子1 (CLCF1) 蛋白质. 这个工具有助于研究CLCF1.

科学领域:

免疫学免疫学免疫学
细胞因子生物学细胞因子生物学
流动细胞计量 (Flow Cytometry) 是一种流动细胞计.

背景情况:

心脏类型细胞因子1 (CLCF1),IL-6家族成员,表现出神经营养和免疫调节的作用.
CLCF1mRNA在淋巴体器官中发现,与T辅助17细胞分化有关.
免疫细胞中CLCF1的蛋白质水平表达数据有限.

研究的目的:

开发一种可靠的方法来检测免疫细胞中的CLCF1蛋白表达.
用流式细胞计量来描述CLCF1在小鼠免疫细胞中的表达.
为了研究CLCF1特异性单克隆抗体的功能影响.

主要方法:

开发一种针对CLCF1.1.的单克隆抗体 (mAb).
通过流细胞测量检测人类和小鼠CLCF1蛋白.
使用CLCF1-表达的Ba/F3细胞和条件淘汰小鼠模型进行验证.

主要成果:

在转导的Ba/F3细胞和小鼠脏T细胞中成功检测到CLCF1蛋白,包括体外分化的Th1细胞.
反CLCF1 mAb在体外显示出对CLCF1的抑制活性,结合到一个关键的表位.
使用来自Clcf1缺陷小鼠的淋巴细胞确认了特异性.

结论:

使用特定mAb的流细胞计是检测CLCF1蛋白表达的可行方法.
这种技术可以研究CLCF1在各种免疫细胞类型中的表达.
开发的方法将推动研究CLCF1在免疫反应中的作用.

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相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...