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阿斯巴酸β-基酶和低氧标记在头癌中的表达:对HPV相关瘤的影响

Jana Smahelova¹, Barbora Pokryvkova¹, Eliska Stovickova¹

¹Department of Genetics and Microbiology, Faculty of Science BIOCEV, Charles University, Prague, Czech Republic.

Infectious agents and cancer

|June 10, 2024

概括

在头癌中,高水平的缺氧标志物如ASPH,HIF1A和GLUT1可能表明HPV感染,这是一个积极的预后因素. 增加HIF1A和GLUT1表达也预测更好的生存率,无论HPV状态.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症研究癌症研究

背景情况:

头部和部状细胞癌 (HNSCCs) 可能是HPV诱导的,与更好的结果相关.
瘤缺氧在HNSCC中很常见,通常与预后不佳和瘤攻击性增加有关.
阿斯巴-β-基酶 (ASPH) 是一种对缺氧反应的基因,涉及瘤生长和转移.

研究的目的:

研究与HPV状态相关的HNSCC中ASPH和缺氧标志物的表达.
为了确定这些标记在HNSCC患者的预后意义.

主要方法:

使用实时PCR和多重光免疫组织化学分析HNSCC样本.
在瘤组织中评估ASPH,HIF1A,GLUT1和MMP13表达.
统计分析包括考克斯比例危险模型来评估预后价值.

主要成果:

在HPV阳性HNSCC中观察到ASPH,HIF1A,GLUT1和MMP13的较高蛋白质表达,与HPV阴性HNSCC相比.
在HPV阴性HNSCC中,EPAS1mRNA表达率较高.
增加的HIF1A和GLUT1蛋白水平被确定为整体和疾病特异性存活的积极预后标志物,独立于HPV状态.

结论:

在HNSCC中增加的ASPH和缺氧标志物可能表明HPV感染,这是一个强烈的积极预后因素.
HIF1A和GLUT1表达可以作为有价值的预后指标,特别是当HPV状态不确定时.

关键词:

亚斯巴酸盐-β-氧化酶头癌是头部和部的癌症.人类乳头瘤病毒人类乳头瘤病毒氧气过低是因为缺氧.预测预后预后

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