探索适用于丸数字病理研究的组织固定方法
Pengxiang Tian1, Zhan Yang1, Changbao Qu1
1Department of Urology, The Second Hospital of Hebei Medical University, 215 Heping W Rd, Shijiazhuang, 050000, China.
European journal of medical research
|June 10, 2024
概括
修改后的戴维森液 (mDF) 固定12小时,为男性生殖发育研究提供最佳的丸组织保存. 这种方法提高了数字病理学的组织病理学部分质量.
科学领域:
- 生殖生物学 生殖生物学
- 组织病理学 组织病理学
- 数字病理学数字病理学
背景情况:
- 丸组织的固定对于保持结合组织和精卵管之间的结构完整性至关重要.
- 高质量的组织病理学断片对于研究男性生殖发育至关重要.
- 数字病理学技术需要为高级分析做好最佳组织准备.
研究的目的:
- 确定优质小鼠丸组织病理学切片的最佳固定和固定持续时间.
- 用数字病理学为深入的男性生殖发育研究奠定基础.
主要方法:
- 用10%的中性缓冲甲 (10%的NBF),修饰的戴维森液体 (mDF) 或布恩液体 (BF) 固定小鼠丸8小时,12小时或24小时.
- 组织学评估涉及Hematoxylin和eosin (H&E) 和周期性酸 Schiff-hematoxylin (PAS-h) 染色.
- 免疫组织化学 (IHC) 用于蛋白质保存的评估,通过Aperio ScanScope CS2进行定量分析.
主要成果:
- 10%的NBF固定减少了15-17%的种子表皮厚度.
- 与10%的NBF相比,BF和mDF表现出更优异的PAS-h染色对宏体和整体形态的染色.
- mDF显示了比BF更好的IHC染色对突触膜复合物3 (Sycp3);长时间固定增加了形态损伤.
结论:
- 布恩的流体 (BF) 呈现出较低的IHC染色效率.
- 经过12小时修改的戴维森液体 (mDF) 固定,产生了出色的形态细节,PAS-h染色用于精卵管分阶段,以及IHC结果.
- 最佳的固定是至关重要的,因为延长的持续时间会对丸组织形态产生负面影响.
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