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相关概念视频

Focusing of Light in the Eye01:16

Focusing of Light in the Eye

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Light rays enter the eye through the cornea, a transparent dome-shaped tissue that is the eye's outermost layer. The cornea bends or refracts, light rays traveling to the pupil. The shape of the cornea determines how much of the light is bent and whether the image will be focused correctly on the retina at the back of the eye. Once the light has passed through both refraction layers, it converges into a single focal point onto a small area. This is where photoreceptors start transforming...
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Author Spotlight: Advancements in Refractive Surgical Correction for Presbyopia and Exploring Postoperative Visual Acuity
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基于纹的主动焦点稳定,具有通用客观镜头兼容性,扩展操作范围和纳米精度.

Amir Rahmani, Tabitha Cox, Akhila Thamaravelil Abhimanue Achary

    Optics express
    |June 11, 2024
    PubMed
    概括

    我们开发了一种精确的,广泛的焦点稳定方法,使用光显微镜的光学. 这种可适应的系统可增强各种显微镜的长期高分辨率成像.

    科学领域:

    • 显微镜和成像科学 显微镜和成像科学
    • 光学物理学 光学物理学

    背景情况:

    • 准确的焦点稳定对于高分辨率的长期光成像至关重要.
    • 使用信托标记或光束反射的现有方法的范围和适用性有限.

    研究的目的:

    • 为显微镜引入一种新,精确和广泛适用的焦点稳定技术.
    • 克服当前多式联接和大规模成像中焦点稳定方法的局限性.

    主要方法:

    • 开发了一种基于光束的焦点稳定方法,利用了纹.
    • 评估了该系统与各种客观镜头 (10x-100x) 的兼容性,以及其精度 (<10 nm) 和操作范围 (>10 μm).
    • 展示了在Raspberry Pi架构上的实现.

    主要成果:

    • 基于的方法在>10μm范围内实现了10nm以下的精度.
    • 该技术证明了与各种客观镜头的兼容性.
    • 系统被证明基本上不受指向稳定性错误的影响.

    结论:

    • 与现有方法相比,开发的基于纹的焦点稳定提供了更高的精度和操作范围.
    • 它的多功能性和与各种客观镜头和显微镜设置的兼容性使其成为光成像的宝贵补充.

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  • 独立的Raspberry Pi实现提供了一个可访问和适应的焦点稳定解决方案.