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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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相关实验视频

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Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
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Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

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深度学习增强的快照超光谱共聚焦显微镜成像系统.

Shuai Liu, Wenzhen Zou, Hao Sha

    Optics express
    |June 11, 2024
    PubMed
    概括

    本研究介绍了一种快照超光谱共聚焦显微镜成像系统 (SHCMS),用于更快的3D成像. 新方法在一次拍摄中实现了高对比度的高光谱图像,显著改善了光谱通道计数和生物样本的成像速度.

    科学领域:

    • 光学和光子学 在光学和光子学.
    • 生物医学成像技术 生物医学成像技术
    • 频谱学是一种光谱学.

    背景情况:

    • 激光扫描共聚焦高光谱显微镜提供了详细的3D样本分析,但由于缓慢的机械扫描而受到限制.
    • 现有的方法难以快速获取高光谱分辨率的3D数据.

    研究的目的:

    • 开发一种新的快照超光谱共聚焦显微镜成像系统 (SHCMS),用于高速3D成像.
    • 为了克服传统激光扫描共聚焦超光谱显微镜的速度限制.

    主要方法:

    • 使用数字微镜装置 (DMD) 集成编码照明显微镜.
    • 开发一个快照超光谱共聚焦神经网络 (SHCNet),用于一次性图像重建.
    • 使用光学切割来实现高效的高光谱体积成像.

    主要成果:

    • 在一次拍摄中实现了高对比度的160频带对焦高光谱图像的土豆的自发光.
    • 与以前的方法相比,光谱通道的改善几乎是5倍.
    • 成功记录了高分辨率和对比度的超光谱体积数据.

    结论:

    • SHCMS在超光谱显微镜的成像速度和光谱分辨率方面取得了重大进展.

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  • 这项技术可实现高效的3D高光谱成像,克服以前的速度瓶.
  • 开发的方法具有实时,高度多重化的生物成像应用的潜力.