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Updated: Jun 24, 2025

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活细胞单分子追踪数据对具有不同光物理性质的光体进行了强大的量化

Amy N Moores1, Stephan Uphoff1

  • 1Department of Biochemistry, University of Oxford, South Parks Rd, Oxford OX1 3QU, U.K.

The journal of physical chemistry. B
|June 11, 2024
PubMed
概括

活细胞单分子追踪使用光体 (TMR,JFX650) 来研究蛋白质的行为. 我们的方法确保从平均平方位移 (MSD) 和隐藏马尔科夫建模 (HMM) 分析中得到一致的结果,无论光体的选择如何.

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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