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Updated: Jun 24, 2025

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在心脏细胞中TRIM35 Monoubiquitinates H2B,对心力衰竭的影响

Maria Areli Lorenzana-Carrillo^1,2,3, Saymon Tejay^1,2,3, Joseph Nanoa^1,2,3

¹Department of Medicine (M.A.L.C., S.T., J.N., Y.L., A.H., Y.Y.Z., E.D.M., G.S.), University of Alberta, Edmonton, Canada.

Circulation research

|June 11, 2024

概括

TRIM35 E3-ubiquitin结合酶单双基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基这种TRIM35和基因素修饰预测了扩张性心肌病变的发病.

科学领域:

心血管生物学心血管生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子心脏病学分子心脏病学

背景情况:

转录因子P53在心力衰竭中被激活,但其在成熟心肌细胞中的诱导机制尚不清楚.
了解非复制性心肌细胞中的P53调节对于心力衰竭研究至关重要.

研究的目的:

在成熟心肌细胞中阐明协调P53诱导与促进剂可访问性的机制.
研究TRIM35在P53调节和心力衰竭中的作用.

主要方法:

使用了TRIM35过度表达和初级心肌细胞培养的转基因小鼠.
采用分子生物学技术,包括ChIP测序,qPCR和RNA测序.
分析了人类扩张性心肌病和非失败的左心室样本.

主要成果:

在成熟心肌细胞中,TRIM35在K120处直接单双化基素2B.
这种表观遗传修饰增强了P53结合和转录的染色质可访问性.
在心肌细胞中,TRIM35诱导的P53活性引发心力衰竭,反映了人类扩张性心肌病.

结论:

TRIM35和K120-ubiquitinated histone 2B是心肌细胞中的关键表观遗传调节剂.
这些分子特征可以预测扩张性心肌病的发展.

关键词:

灭症 (apoptosis) 是一种死亡的过程.在表观基因组学上,表观基因组学.心脏衰竭是因为心脏衰竭.基因组基因组基因组

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These groups modify specific amino acids in a protein....