分离和净化方法对格力克多糖的抗氧化,低血糖和DNA保护活性的影响
Haiyang Wang1, Yingjie Feng2, Yifan Liang1
1College of Tobacco Science, Henan Agricultural University, Zhengzhou, 450046, China.
Chemistry & biodiversity
|June 11, 2024
概括
使用不同的方法净化了低分子量公多糖 (FP). 这些纯化的FP表现出抗氧化剂,低血糖和DNA保护作用,其中一个部分显示出最高的疗效.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
背景情况:
- 草多糖 (FP) 以其潜在的健康益处而闻名.
- 优化隔离和净化方法对于最大限度地提高FP生物活性至关重要.
研究的目的:
- 为了比较乙醇逐步沉和DEAE-52纤维素色谱对格力克多糖的特性和生物活性的影响.
- 为了确定 fenugreek 多糖的最佳净化方法.
主要方法:
- 使用乙醇分级沉和DEAE-52纤维素柱色谱,分离和净化六种低分子量公多糖分.
- 聚糖的特性,包括总糖含量,结构分析 (银糖与曼诺糖的比率,α-D-银酸和β-D-曼诺酸的结构) 和分子重量的确定 (4-19 kDa).
- 评估生物活动,包括抗氧化剂,低血糖和DNA保护作用.
主要成果:
- 与乙醇沉分数相比,DEAE-52的纤维素化分数具有更高的总糖含量和更松散的结构.
- 所有六种FP分离物都表现出抗氧化,低血糖和DNA保护作用.
- 通过DEAE-52纤维素染色学净化DFP-0部分显示出最显著的生物活性.
结论:
- 不同的隔离和净化技术显著改变了格力克多糖的物理化学特性和生物活性.
- DEAE-52纤维素柱法,特别是DFP-0分数,对于获得具有增强生物活性的青菜多糖类是有效的.
- 这些发现为 fenugreek 多糖在功能性食品和药品中的应用提供了理论基础.
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