Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA as a Genetic Template02:05

DNA as a Genetic Template

21.9K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
21.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Large-Scale Collaborative Assessment of Binding Free Energy Calculations for Drug Discovery Using OpenFE.

Journal of chemical information and modeling·2026
Same author

An Automated Workflow for Diagnosing Sampling Issues Caused by Slow Torsional Motions in Molecular Simulations.

Journal of chemical information and modeling·2026
Same author

Efficient Binding Affinity Estimation for Fragment-Based Compounds Using a Separated Topologies Approach.

Journal of chemical information and modeling·2026
Same author

The Open Molecular Software Foundation (OMSF) and the Growing Role of Open Source Software in Molecular Modeling.

Journal of chemical information and modeling·2026
Same author

A Quadrupolar Fullerene Model System for Benchmarking Enhanced Sampling of Trapped Waters in Free Energy Calculations.

The journal of physical chemistry. B·2026
Same author

A simple compound prioritization method for drug discovery considering multi-target binding.

Digital discovery·2026
Same journal

PACEff Builder: An Efficient Platform for Constructing PACE Hybrid-Resolution Models for Molecular Dynamics Simulations of Aqueous Protein, Peptide Assembly, and Membrane Protein Systems.

Journal of chemical information and modeling·2026
Same journal

TransKla: A Local-Global Cross-Attention Based Transformer Approach for Prediction of Lysine Lactylation Sites.

Journal of chemical information and modeling·2026
Same journal

CondenSimAdapter: A Versatile Builder for Multiscale Simulations of Protein Condensates with Broad Force-Field Compatibility and Robust Dense-Phase Relaxation.

Journal of chemical information and modeling·2026
Same journal

Simulation Guided Design of a Potentially Hyperactive Ice Nucleating Protein.

Journal of chemical information and modeling·2026
Same journal

Setting the Bases of the Photogenotoxicity of <i>p</i>-Aminobenzoic Acid.

Journal of chemical information and modeling·2026
Same journal

Probing Charge-Controlled Inter-Domain Flexibility: Integrating Experimental and Coarse-Grained Approaches.

Journal of chemical information and modeling·2026
查看所有相关文章

相关实验视频

Updated: Jun 24, 2025

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
11:22

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

18.1K

对DNA编码图书馆设计的建构块为中心的方法.

Patrick R Fitzgerald1, Anjali Dixit2, Chris Zhang3

  • 1Skaggs Doctoral Program in the Chemical and Biological Sciences, Scripps Research, La Jolla, California 92037, United States.

Journal of chemical information and modeling
|June 11, 2024
PubMed
概括
此摘要是机器生成的。

用DNA编码的图书馆为药物发现提供了广泛的化学探索. 最佳设计侧重于各种构建块,如氨基和酸,而不是成本,以最大限度地覆盖化学空间.

更多相关视频

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

10.6K
gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
08:15

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

12.3K

相关实验视频

Last Updated: Jun 24, 2025

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
11:22

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

18.1K
Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples
13:26

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

Published on: April 17, 2015

10.6K
gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
08:15

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

12.3K

科学领域:

  • 药用化学 医学化学
  • 药物发现技术 药物发现技术
  • 化学生物学 化学生物学

背景情况:

  • DNA编码图书馆 (DEL) 技术为药物发现提供了大量的化学结构空间.
  • 设计有效的DEL需要平衡物理化学性质 (PCP),结构多样性和实际合成约束.
  • 了解组合图书馆设计,包括化学循环和构建块 (BB) 选择,对于成功导航至关重要.

研究的目的:

  • 分析DEL中的组合图书馆设计约束,以实现最佳的图书馆生成.
  • 为了比较两个循环的DEL设计 (氨基酸 + 碳酸,初级氨基 + 碳酸) 关于PCP和化学空间覆盖.
  • 评估构建块选择策略和约束对DEL设计的影响.

主要方法:

  • 对组合图书馆设计约束的分析:化学循环数,键构建策略和BB类选择.
  • 使用氨基酸 + 碳酸和初级氨基 + 碳酸构建块的双循环DEL设计的比较.
  • 在不同的BB选择策略和约束条件下评估物理化学性质 (PCP) 和化学空间覆盖.

主要成果:

  • 氨基和碳酸构建块的广泛可用性对于探索最广的化学空间至关重要.
  • 成本不是化学太空探索的主要驱动因素;类似的化学空间可以使用更便宜的构建块来访问.
  • 两周期图书馆设计表明,BB类的选择显著影响PCP和多样性.

结论:

  • 战略性选择多样化和易于获得的构成块,特别是氨基和酸,是最大限度地利用DEL中的化学空间的关键.
  • 德尔的设计应该优先考虑BB的多样性,而不是成本,以实现最佳的化学空间覆盖和药物发现潜力.
  • 这些发现为设计高效和有效的DNA编码库提供了实际指导.