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在Anabantoid鱼类中,对长波长敏感的Opsin的扩张和功能多样化

Jan Gerwin^1,2, Julián Torres-Dowdall^3,4, Thomas F Brown^5,6

¹Zoology and Evolutionary Biology, Department of Biology, University of Konstanz, Konstanz, Germany.

Journal of molecular evolution

|June 11, 2024

概括

贝塔鱼的基因重复导致了新的红色敏感素 (lws) 基因. 这些重复的基因进化了不同的功能,可能通过发育过程中的表达变化来增强视力.

科学领域:

进化生物学是进化的生物学.
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

基因重复是遗传和表型多样性的主要驱动力.
视觉素基因进化,特别是远鱼,以频繁的重复和功能多样化为特征.
虽然对绿色敏感的素 (rh2) 复制在鱼类中很常见,但对多种红色敏感的素 (lws) 复制的理解较少.

研究的目的:

为了研究 anabantoid 鱼类,特别是 Betta. 属的红敏感 opsin (lws) 基因的进化史和功能多样化.
为了确定贝塔血统中lws基因重复的数量和时间.
探索lws基因复制的功能后果,包括蛋白质序列和基因表达的变化.

主要方法:

全基因组测序九种阿纳班托类物种,包括Betta imbellis的基因组.
遗传学分析以确定lws基因的进化关系.
氨基酸序列分析,以确定lws对应物中的功能分歧.
在Betta splendens的眼睛转录学研究在发育过程中素基因表达模式.

主要成果:

在贝塔血统中,至少发生了两种lws opsin基因的独立重复.
在Betta lws paralogs的关键跨膜区域观察到显著的氨基酸序列多样化.
在关键调点预测的氨基酸替代表明lws对应物中变化的光谱灵敏度 (λmax).
转录基因分析显示,在Betta splendens的不同发育阶段,lws对应物之间存在表达转移.

结论:

贝塔的多个lws基因重复导致了功能多样化,由序列变化和改变的光谱调表明.
在lws基因表达的时间对线性,安排在一个集群中,可能促进了亚功能化.
这项研究提供了关于远鱼视觉系统演化的见解,突出了基因重复和随后的多样化在适应性进化中的作用.

关键词:

基因复制是基因的复制.基因表达基因表达基因表达在Opsin基因基因中.罗族的战斗鱼是什么意思

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