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Updated: May 12, 2026

Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays
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Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays

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使用原始叶子提取物和重组酶来确定动力参数Km和Vmax的高通量糖酸酸活性试验,使用微板阅读器来确定动力参数Km和Vmax

Ludmila V Roze1, Audrey Johnson1, Luke M Gregory2

  • 1Department of Energy - Plant Research Laboratory, Michigan State University, East Lansing, MI, USA.

Methods in molecular biology (Clifton, N.J.)
|June 11, 2024
PubMed
概括

这项研究引入了一种快速,经济高效的96孔微板测定方法,用于测量植物中的糖酸酸酶 (PGLP) 活性. 这种方法简化了用于大规模查和动力学研究的酶分析.

关键词:
缓冲器准备应用程序缓冲器准备应用程序Km和Vmax计算应用程序的应用程序这就是Menten Menten.迈克尔·米卡利斯 (Michael Michaelis) 是一个著名的作家.糖酸酸盐酸酶是一种酸.摄影呼吸是一种光学呼吸.再组合糖酸酸酶的含量是多少

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相关概念视频

Enzyme Kinetics01:19

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...