相关实验视频

Updated: Jun 24, 2025

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases

Published on: February 24, 2018

用于测量氧酸降解酶活性的光谱测试

Aaron H Liepman¹, Mara Jaworski², Citlalxochilt Ramirez-Lopez²

¹Biology Department, Eastern Michigan University, Ypsilanti, MI, USA. aliepman@emich.edu.

Methods in molecular biology (Clifton, N.J.)

|June 11, 2024

概括

氧酸还原酶 (HPR) 是光呼吸的关键. 这项研究提出了一种光谱光度测试,通过跟踪NADH消耗来测量HPR活性,以帮助光呼吸研究.

科学领域:

生物化学生物化学
植物生理学植物生理学

背景情况:

氧酸还原酶 (HPR) 是光呼吸道中一个关键的酶.
HPR催化了氧酸降解为糖酸盐,利用NADH.
这种反应对于植物的碳代谢和同化至关重要.

研究的目的:

开发和详细说明可靠的光谱测量试验,以量化HPR活动.
提供一种用于体外测量HPR酶动态的方法.

主要方法:

谱光度分析监测340nm的NADH消耗.
在体外进行的酶定量.

主要成果:

该试验通过检测由于NADH氧化而在340nm的吸收率下降,成功测量了HPR活性.
对HPR活动的量化是可以实现的.

结论:

描述的光谱测量试验是研究光呼吸中的HPR功能的一个有价值的工具.
这种方法有助于对HPR及其在植物新陈代谢中的作用进行生化表征.

关键词:

酶动力学酶动力学氧酸可以减少脱酶.它们是NADH和NADH.摄影呼吸是一种光学呼吸.用光谱光度测试进行测试.

更多相关视频

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

Published on: March 18, 2012

Biochemical Measurement of Neonatal Hypoxia

Biochemical Measurement of Neonatal Hypoxia

Published on: August 24, 2011

相关概念视频

Measuring Reaction Rates

Measuring Reaction Rates

Polarimetry finds application in chemical kinetics to measure the concentration and reaction kinetics of optically active substances during a chemical reaction. Optically active substances have the capability of rotating the plane of polarization of linearly polarized light passing through them—a feature called optical rotation. Optical activity is attributed to the molecular structure of substances. Normal monochromatic light is unpolarized and possesses oscillations of the electrical...