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Updated: Jul 31, 2026

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET
13:38

Monitoring Kinase and Phosphatase Activities Through the Cell Cycle by Ratiometric FRET

Published on: January 27, 2012

使用基于FRET的生物传感器测量细胞周期Zn2+动态的协议

Samuel E Holtzen1, Ananya Rakshit2, Amy E Palmer2

  • 1Molecular, Cellular & Developmental Biology, University of Colorado Boulder, Boulder, CO 80309, USA; Department of Biochemistry and BioFrontiers Institute, 3415 Colorado Avenue, University of Colorado Boulder, Boulder, CO 80303, USA.

STAR protocols
|June 11, 2024
PubMed
概括

这项研究详细介绍了一种长期活细胞成像方法,以追踪细胞内的 (Zn2+) 波动. 该协议允许研究人员观察细胞水平如何自然变化以及对外部因素的反应.

关键词:
细胞生物学 细胞生物学显微镜的使用方法一个单细胞的单细胞.

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...