在Saccharomyces cerevisiae中改善贝宁生产的酶和通路工程
Jiawei Li1,2, Lemin Wang1,2, Nan Zhang1,2
1Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Yaguan Road 135, Jinnan District, Tianjin 300350, China.
ACS synthetic biology
|June 11, 2024
概括
在酵母中,微生物生产天然的红色素贝塔宁显著增强. 机械酵母实现了创纪录的134.1 mg/L贝塔宁标位,为甜菜提取提供了一个可持续的替代方案.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 贝塔宁是一种天然的红紫色素,来自贝塔素,作为食品染色剂和健康益处而受到重视.
- 目前通过采摘甜菜根的商业贝坦因生产面临经济和可持续性的局限性.
- 微生物异质生产提供了一个可行的和可持续的替代方案.
研究的目的:
- 为了改进*Saccharomyces cerevisiae*以改进*de novo*贝素的生产.
- 为了增强植物P450酶的催化活性,这些酶参与贝塔宁生物合成.
- 为了优化前体代谢以增加贝他宁产量.
主要方法:
- 使用计算模拟和分子对接的植物P450酶 (CYP76AD) 的组合工程.
- 半导体设计,氨酸替代和局部定向和突变发生,以改善酶.
- 代谢工程来增强l-铁素路径流量和UDP-葡萄糖供应.
- 在Saccharomyces cerevisiae*中优化发酵过程.
主要成果:
- 与野生类型相比,CYP76AD的组合突变表现出比坦标位增加了约7倍.
- 增强的l-氨酸通路流量和UDP-葡萄糖供应进一步促进了贝他宁的产生.
- 改造品种BEW10在糖糖的摇瓶中实现了创纪录的134.1 mg/L的贝坦宁标位.
结论:
- P450酶和代谢工程策略对于有效的微生物生产贝他宁是有效的.
- 工程*Saccharomyces cerevisiae*为可持续的贝生产提供了一个有前途的平台.
- 这项研究为微生物贝塔宁标位建立了新的基准,为工业应用铺平了道路.
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