尿生物功能化等离子体传感器用于检测尿酸,使用APTES修饰的金纳米顶层
Olabisi Abdullahi Onifade1, Fatin Adriena Yusairie2, Muhammad Hafiz Abu Bakar1
1Department of Computer and Communication Systems Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia; Wireless and Photonics Research Centre of Excellence, Faculty of Engineering, Universiti Putra Malaysia, 43400, Serdang Selangor, Malaysia.
Biosensors & bioelectronics
|June 11, 2024
概括
这项研究开发了一种高度敏感的等离子体传感器,用于在护理点检测尿酸. 这种生物功能传感器具有出色的选择性,并有可能彻底改变临床诊断.
科学领域:
- 生物感知和纳米技术
- 生物医学工程 生物医学工程
- 分析化学 分析化学
背景情况:
- 目前的尿酸检测方法缺乏敏感性和选择性,不能在医院使用.
- 血传感器是有前途的,但在临床应用中需要提高性能.
研究的目的:
- 开发一种使用表面等离子体共振的生物功能化传感器,以量化生理相关度的尿酸.
- 提高用于尿酸检测的等离子体传感器的灵敏度和选择性.
主要方法:
- 用EDC/NHS交联剂将尿酶对传感器探针进行共价固定.
- 使用原子力显微镜和里埃变换红外光谱学的表征.
- 使用兰格穆尔吸附异温模型分析结合动力学.
主要成果:
- 传感器显示了高的结合亲和力 (298.83 (mg/dL) -1) 和灵敏度 (0.0755°/(mg/dL)).
- 实现了低检测极限 (0.095 mg/dL) 和对常见干扰物的优良选择性.
- 对于0.5mg/dL尿酸,观察到0.3706°的明显共振角度偏移,使其与干扰物区分开来.
结论:
- 生物功能化等离子体传感器显著提高了尿酸检测能力.
- 这项技术对临床诊断和护理点应用具有前景,有可能改变医疗保健结果.
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