相关实验视频

Updated: Jun 24, 2025

Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility

Generation of Null Mutants to Elucidate the Role of Bacterial Glycosyltransferases in Bacterial Motility

Published on: March 11, 2022

O-GlcNAc循环酶的非催化域为功能特定控制提供了新的机会

Chia-Wei Hu¹, Ke Wang¹, Jiaoyang Jiang¹

¹School of Pharmacy, University of Wisconsin-Madison, 777 Highland Ave, Madison, WI 53705, USA.

Current opinion in chemical biology

|June 11, 2024

概括

一个关键蛋白质修饰的O-GlcNAcylation是由O-GlcNAc转移酶 (OGT) 和O-GlcNAcase (OGA) 调节的. 针对它们的非催化领域,为控制疾病中的O-GlcNAcylation提供了新的策略.

科学领域:

生物化学和分子生物学
葡萄糖生物学葡萄糖生物学
酶学是一种酶学.

背景情况:

O-GlcNAcylation是一种动态的翻译后修饰,对细胞信号传输至关重要,由O-GlcNAc转移酶 (OGT) 和O-GlcNAcase (OGA) 调节.
失调的O-GlcNAcylation与许多疾病有关,包括癌症,糖尿病和神经退行性疾病.
治疗开发的挑战来自大量的O-GlcNAcylated蛋白质和OGT和OGA复杂的基质识别机制.

研究的目的:

审查了解OGT和OGA非催化领域的结构和机制的最新进展.
突出这些非催化领域在调节酶功能和基质特异性的关键作用.
通过针对这些领域来探索调节O-GlcNAcylation的新疗法机会.

主要方法:

关于OGT和OGA非催化领域的最新结构和机制研究的文献综述.
对非催化域在蛋白质-蛋白质相互作用和基质选择中的作用的新兴证据的分析.
讨论目前和潜在的工具来调查和准这些领域.

主要成果:

OGT和OGA的非催化域是它们酶活性和基质特异性的关键调节者.
这些域调解关键的蛋白质-蛋白质相互作用,影响O-GlcNAcylation模式.
结构和机制洞察力揭示了针对性调制的潜在地点.

结论:

针对OGT和OGA的非催化领域,为开发针对异常O-GlcNAcylation相关疾病的特定疗法提供了一个有希望的途径.
了解这些域的功能对于精确控制O-GlcNAcylation路径至关重要.
对这些领域的结构和机制的进一步研究将有助于开发新的治疗策略.

关键词:

特定功能的调制器.非催化领域的领域.O-GlcNAc转移酶 (OGT) 是一种在O-GlcNAcase (OGA) 中使用.

更多相关视频

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

Published on: October 4, 2017

Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells

Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells

Published on: April 27, 2010

相关概念视频

GPCRs Regulate Adenylyl Cylase Activity

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...

The Citric Acid Cycle: Output

The Citric Acid Cycle: Output

The citric acid cycle is termed an amphibolic pathway as it operates both anabolically and catabolically. The cyclic reactions balance the flux of the substrates to provide an optimal concentration of NADH and ATP to the cell.
Regulation of Citric Acid Cycle
The citric acid cycle is regulated in several ways, including feedback inhibition, regulation of enzyme activities, and associated anaplerotic or cataplerotic pathways.
The primary substrate of the TCA cycle—acetyl CoA—is...

Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Allosteric Proteins-ATCase

Allosteric Proteins-ATCase

Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...

What is Glycolysis?

What is Glycolysis?

Overview
Cells make energy by breaking down macromolecules. Cellular respiration is the biochemical process that converts "food energy" (from the chemical bonds of macromolecules) into chemical energy in the form of adenosine triphosphate (ATP). The first step of this tightly regulated and intricate process is glycolysis. The word glycolysis originates from the Latin glyco (sugar) and lysis (breakdown). Glycolysis serves two main intracellular functions: generating ATP and generating...

Regulated Protein Degradation

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...