蛋白质的终端区域是低复杂度区域和选择的热点
Lokdeep Teekas1, Sandhya Sharma1, Nagarjun Vijay1
1Computational Evolutionary Genomics Lab, Department of Biological Sciences, IISER Bhopal , Bhauri, Madhya Pradesh, India.
Open biology
|June 11, 2024
概括
挥发性低复杂性区域 (LCRs) 驱动着四足动物的适应性变异和进化新奇性. 这些LCRs通常在基因终端发现,尽管促进了快速的功能多样化,但它们仍处于强烈的净化选择之下.
科学领域:
- 进化生物学 进化生物学
- 基因组学就是基因组学.
- 分子进化分子进化
背景情况:
- 挥发性低复杂性区域 (LCR) 越来越被认为是适应性变异,功能多样化和进化新奇性的重要贡献者.
- 自然选择和突变动态之间的相互作用对于塑造LCR的组成和长度至关重要,复制滑动等机制影响长度变化.
- 需要更深入地了解如何选择和突变共存来调节LCRs.
研究的目的:
- 为了研究基因组分布和低复杂度区域 (LCRs) 在四足类中的进化作用.
- 确定LCRs,积极选择位 (PSS) 和基因功能之间的关系.
- 阐明LCRs对进化压力的影响,特别是净化选择及其组成依赖.
主要方法:
- 跨四足动物类的比较基因组分析.
- 在基因结构中识别和定位低复杂度区域 (LCR) 和正选择位点 (PSS).
- 计算和分析进化速率,包括omega (dN/dS),以推断选择压力.
主要成果:
- 低复杂性区域 (LCRs) 和积极选择的位点 (PSS) 偏好地定位到大多数四足动物群中的基因末端区域.
- 含有LCR的基因表现出明显更高的GC含量和更低的欧米茄值,这表明尽管促进了快速的功能多样性,但仍有强烈的净化选择.
- LCRs在基因内显示一种位置偏好,这取决于基因的纯度,这表明基因的组成驱动着进化作用.
结论:
- 积极选择的地点 (PSS) 具有特定位置的角色,其中中央PSS参与防御和终端PSS参与非特定功能.
- 低复杂度区域 (LCR) 经历了强烈的净化选择,平衡它们在产生功能多样性的作用.
- 这些发现共同增强了我们对选择和LCRs如何相互作用以塑造四足动物的遗传多样性和适应性的理解.
更多相关视频
相关概念视频
Conserved Binding Sites
4.2K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.2K
Conservation of Protein Domains Over Different Proteins
10.8K
Protein domains are small structurally independent units that are part of a single amino acid chain. Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
10.8K
Gene Evolution - Fast or Slow?
7.1K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
7.1K
Protein Networks
3.9K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.9K
Regulated mRNA Transport
6.3K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.3K
The Eukaryotic Promoter Region
16.3K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
16.3K


