含有Plasmodium LCCL域的模块化蛋白质起源于祖先的膜体
Callum De Hoest-Thompson1, Virginia Marugan-Hernandez1, Johannes T Dessens2
1The Royal Veterinary College, Hawkshead Lane , North Mymms AL9 7TA, UK.
Open biology
|June 11, 2024
概括
莱克域粘合剂样蛋白 (LAP) 对于疟疾寄生虫的传播至关重要. 这项研究揭示了LAP是古老的蛋白质,早于Apicomplexa和Alveolata,在各种真核细胞系中保留了角色.
科学领域:
- 分子生物学分子生物学
- 寄生虫学的寄生虫学
- 进化生物学 进化生物学
背景情况:
- 虫寄生虫利用含有L-lectin域 (LCCL) 的lectin域粘合剂样蛋白 (LAPs) 来进行传播.
- LAP是具有复杂架构的模块化蛋白质,对于疟疾寄生虫生命周期至关重要.
- 当前,LAP的进化起源和更广泛的分布在很大程度上仍未被探索.
研究的目的:
- 在真核生物体中研究L-lectin域含有 (LCCL) 莱克域粘合物样蛋白 (LAP) 的进化史和遗传分布.
- 为了确定LAP是否仅限于Apicomplexa,或者它们是否在相关的真核细胞系中更广泛地存在.
主要方法:
- 欧核生物基因组的生物信息分析,以确定含有L-lectin域 (LCCL) 的lectin域粘合物样蛋白 (LAP) 的正义.
- 遗传学分析以追踪LAP基因的进化保存和多样化.
- 进行比较基因组学,以评估LAP目录中的基因丢失和重复事件.
主要成果:
- 在所有Apicomplexa中,正统的L-lectin域含有 (LCCL) 莱克丁域粘着性蛋白样蛋白 (LAP) 基因被保存,由于基因重复和损失而存在谱系特异的变异.
- 此外,LAP还存在于Apicomplexa的最亲密的亲属Chromerids中,它们具有最多样化的谱系,包括一种新的膜结合LCCL蛋白.
- 在恐龙,类动物和类动物中没有LAP,但在类动物的姊妹群体 - - 类动物中发现了LAP,这表明它们的起源很古老.
结论:
- 含L-lectin域的 (LCCL) 莱克丁域粘合剂样蛋白 (LAP) 是古老的蛋白质,早于Apicomplexa和Alveolata的出现.
- 在科尔波尼米德和克罗米里德中存在LAP表明它们的进化起源早于Alveolata超群.
- 了解LAP的广泛进化保护,可以了解疟疾寄生虫传播之外的基本生物过程.
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