作为一种化衍生试剂的4-二尼特作为一种化衍生试剂,用于使用苏子基合反应在全血样本中对L-p-二烯氨酸进行染色学分析

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Takayuki Fukuda¹, Naoya Kishikawa¹, Mahmoud El-Maghrabey²

¹Department of Analytical Chemistry for Pharmaceuticals, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan.

Analytica chimica acta

|June 11, 2024

概括

一种新的HPLC-FL方法准确地测量了血中的L-p-乙烯氨酸 (BPA),用于中子捕获疗法 (BNCT). 这种超敏感的技术使用最小的血液容量,为患者监测提供了比现有的方法更简单的替代方案.

科学领域:

分析化学分析化学
生物医学科学生物医学科学
辐射疗法辐射疗法

背景情况:

对于子中子捕获疗法 (BNCT),一种向癌症治疗,L-p-烯氨酸 (BPA) 是至关重要的.
精确的BPA血液水平测量对于有效的BNCT至关重要.
现有的方法 (ICP-AES,ICP-MS) 是复杂的,昂贵的,需要专家分析师.

研究的目的:

开发一种更简单,更灵敏的方法来确定人类血液中的BPA.
为了利用BPA衍生物化的新型化试剂.
为了能够有效地监测BNCT患者的BPA水平.

主要方法:

作为BPA的新型化试剂,采用了4-IOBENZONITRILE.
使用的苏苏基合器用于BPA的前列衍生.
开发了一种具有光检测 (HPLC-FL) 的高性能液体染色学方法.

主要成果:

形成了一种光的蓝衍生物,在290/335nm时具有最佳的激发/发射.
该方法表现出极好的线性 (r2=0.997) 从0.5-1000nmol/L,低检测极限为0.26nmol/L.
成功应用于人类全血样本,使用仅10μL血液的高回收率 (≥95.7%).

结论:

4-Iodobenzonitrile是一种有效的化试剂,用于通过苏苏基合确定BPA.
开发的HPLC-FL方法可以在全血中提供超敏感的BPA量化.
这种方法在灵敏度上优越,需要最小的样本体积,适合微型血液分析.

关键词:

4 - 二二二二四二二四二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二二血液样本血液样本中子捕获疗法中子捕获疗法的衍生法的衍生法 L-p-Boronophehylalanine的使用方法苏苏基合反应反应

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