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人类牛奶和母乳衍生的细胞外囊泡的氧利平蛋白概况

Abel Albiach-Delgado¹, Jose L Moreno-Casillas², Isabel Ten-Doménech¹

¹Neonatal Research Group, Health Research Institute Hospital La Fe (IIS La Fe), Avda Fernando Abril Martorell 106, 46026, Valencia, Spain; Primary Care Interventions to Prevent Maternal and Child Chronic Diseases of Perinatal and Developmental Origin Network (RICORS-SAMID) (RD21/0012/0015), Instituto de Salud Carlos III, Madrid, Spain; Servicio de Análisis de Vesículas Extracelulares (SAVE), Health Research Institute Hospital La Fe (IIS La Fe), Avda Fernando Abril Martorell 106, 46026, Valencia, Spain.

Analytica chimica acta

|June 11, 2024

概括

这项研究描述了人乳 (HM) 中的氧利平和HM衍生的小细胞外囊泡 (HMEVs). 抗炎性氧利平因被选择性地丰富在HMEV中,这表明婴儿健康的治疗潜力.

科学领域:

利皮多米克 (Lipidomics) 是一种消化剂.
细胞外囊泡生物学细胞外囊泡生物学
新生儿免疫学新生儿免疫学

背景情况:

人母乳中的小细胞外囊泡 (sEV) 对于新生儿免疫发育至关重要.
氧利平,生物活性脂质,以其抗炎作用而闻名.
了解HM氧利平的特征及其在HM衍生的sEV (HMEV) 中的存在至关重要.

研究的目的:

描述人乳 (HM) 和HM衍生的小细胞外囊泡 (HMEVs) 中的氧利平因特征.
调查HMEV中各种氧利平的存在和分布.
探索HMEV封装氧利平在新生儿健康中的潜在作用.

主要方法:

开发和验证一种超高性能液体染色体协同质谱法 (UPLC-MS/MS) 方法.
在15个个体的HM和HMEV分离物中对106种氧利平的量化和半量化.
在HM和HMEV矩阵之间分析明显的氧利平因型.

主要成果:

在HM或HMEV中检测到106种氧利平中的42种,其中38种是两者共同的.
在HM和HMEV中观察到明显的氧利平特征,这表明了特定的封装机制.
在HMEV中,抗炎性多可萨赫萨酸衍生物 (17-HDHA,14-HDHA) 富含,而在HM样本中,富含酸衍生物 (9-10-DiHOME,12,13-DiHOME).

结论:

这项研究提供了第一个证据,证明了HMEV中抗炎性氧利平的选择性丰富.
这些发现表明,HMEVs可能在向新生儿提供抗炎化合物方面发挥重要作用.
未来的研究将探索HMEV封装的氧利平的治疗应用,特别是预防早产婴儿死性肠球炎.

关键词:

细胞外囊泡中的细胞外囊泡.人类的牛奶是人类的牛奶.质谱学 (UPLC-MS/MS) 是一种氧化脂质是氧化的脂质.氧利平素是一种氧利平素.超高性能液体色谱学超高性能液体色谱学

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