Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.8K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Context-aware simulation enables systematic optimization of long-read mapping parameters.

GigaScience·2026
Same author

gSV: a general structural variant detector using the third-generation sequencing data.

Briefings in bioinformatics·2026
Same author

SpatialCOC: an integrative framework for spatial continuous mapping and cross-omics correction in spatial multi-omics data.

Nature communications·2026
Same author

Highly accurate ab initio gene annotation with ANNEVO.

Nature methods·2026
Same author

Population-level structural variant characterization using pangenome graphs.

Nature genetics·2026
Same author

Genome and transcriptome-based identification and expression profiling of chemosensory gene families across developmental stages and tissues in Sirex noctilio (Hymenoptera: Siricidae).

Insect molecular biology·2026

相关实验视频

Updated: Jun 24, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.0K

下一篇 波兰语2:使用HiFi组装的基因组的重复感知抛光工具 长读

Jiang Hu1,2, Zhuo Wang2, Fan Liang2

  • 1School of Automation Science and Engineering, Faculty of Electronic and Information Engineering, Xi'an Jiaotong University, Xi'an 710049, China.

Genomics, proteomics & bioinformatics
|June 11, 2024
PubMed
概括

下一篇Polish2从PacBio HiFi长读取中改进了基因组组,在不引入过度校正或单元型切换的情况下修复了基础错误. 这种工具可以提高端粒对端粒基因组的准确性.

关键词:
纠正错误 纠正错误 纠正错误 纠正错误基因组组装组的基因组组装组基因组抛光 基因组抛光HiFi 长时间阅读端粒到端粒的端粒

更多相关视频

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

3.6K
Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance
04:58

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance

Published on: December 13, 2024

2.3K

相关实验视频

Last Updated: Jun 24, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
12:08

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

5.0K
De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

3.6K
Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance
04:58

Author Spotlight: Investigating the Role of Repetitive DNA Misregulation in Cancer Initiation and Immunotherapy Resistance

Published on: December 13, 2024

2.3K

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • PacBio的高保真 (HiFi) 长读序列推进了基因组组装的准确性.
  • 高频读取仍然包含基层错误,特别是在容易出错的区域.
  • 当前的基因组抛光工具往往会导致过度校正和单基因型切换错误.

研究的目的:

  • 为了介绍NextPolish2,一个升级的基因组抛光工具.
  • 为了解决纠正HiFi长读组件的现有工具的局限性.
  • 为了提高端粒到端粒子 (T2T) 基因组的准确性.

主要方法:

  • 开发NextPolish2,一个增强的基因组抛光算法.
  • 应用NextPolish2来纠正HiFi长读基因组组合中的基础错误.
  • 评估NextPolish2在最大限度地减少过度校正和单元型切换错误方面的表现.

主要成果:

  • 下一篇 波兰语2有效地纠正了HiFi基因组组合中的基本错误.
  • 该工具避免引入过度的过度校正.
  • 下一篇 波兰语2 在抛光过程中最大限度地减少了哈普类型切换错误.

结论:

  • 下一篇Polish2为抛光HiFi长读基因组组件提供了显著的改进.
  • 该工具对于提高T2T基因组的准确性至关重要.
  • 下一个波兰语2是免费的,促进其在基因组研究中的采用.