Jove
Visualize
联系我们

相关概念视频

Transfer RNA Synthesis02:36

Transfer RNA Synthesis

11.9K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
11.9K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

9.3K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.3K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

24.1K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
24.1K
pre-mRNA Processing02:01

pre-mRNA Processing

52.8K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
52.8K
Ribozymes02:47

Ribozymes

11.2K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
11.2K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

13.2K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Perturbation of the circadian rhythmic transcription contributes to radiotherapy-induced salivary gland hypofunction.

Communications biology·2026
Same author

A framework for the exploration of subcellular compartmentalization of RNA-binding proteins.

Nature communications·2026
Same author

S-phase PARylation of microprotein RSMC enhances the function of Sororin in sister chromatid cohesion.

The EMBO journal·2025
Same author

MCM5 UFMylation regulates replication origin firing and fork progression.

The EMBO journal·2025
Same author

DNA damage response regulator ATR licenses PINK1-mediated mitophagy.

Nucleic acids research·2025
Same author

FOXP1 phosphorylation antagonizes its O-GlcNAcylation in regulating ATR activation in response to replication stress.

The EMBO journal·2024
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jun 24, 2025

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
09:16

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Published on: May 3, 2014

12.8K

RNase P:超越了前体tRNA的处理过程

Peipei Wang1, Juntao Lin1,2, Xiangyang Zheng3

  • 1Guangdong Key Laboratory for Genome Stability & Disease Prevention and Marshall Laboratory of Biomedical Engineering, Shenzhen University Medical School, Shenzhen University, Shenzhen 518060, China.

Genomics, proteomics & bioinformatics
|June 11, 2024
PubMed
概括
此摘要是机器生成的。

核酶P (RNase P) 对于tRNA成熟至关重要,并且在DNA修复和基因组稳定性中也起作用. 这些非正典功能表明RNase P是癌症治疗的潜在治疗标.

关键词:
染色素组合组件的组合对DNA损伤的反应反应基因组稳定性 基因组稳定性在RNase P中使用RNase P瘤发生的起源

更多相关视频

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo
10:51

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo

Published on: October 31, 2014

27.1K
Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach
12:12

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach

Published on: March 12, 2017

9.8K

相关实验视频

Last Updated: Jun 24, 2025

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
09:16

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Published on: May 3, 2014

12.8K
Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo
10:51

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo

Published on: October 31, 2014

27.1K
Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach
12:12

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on TRO Approach

Published on: March 12, 2017

9.8K

科学领域:

  • 分子生物学分子生物学
  • 生物化学 生物化学
  • 遗传学 是一个遗传学.

背景情况:

  • 核酶P (RNase P) 传统上是用于tRNA处理的.
  • 新出现的证据揭示了超出RNA催化作用的非正规功能.
  • 人们越来越认识到RNase P在细胞过程中的作用.

研究的目的:

  • 审查了解RNase P的非正规功能方面的最新进展.
  • 探索RNase P在染色体组合和DNA损伤反应中的参与.
  • 讨论对基因组稳定性,瘤发生和癌症疗法的影响.

主要方法:

  • 关于RNase P. 的最新研究的文献综述
  • 对RNase P在DNA修复和基因组维护中的作用的研究分析.
  • 综合与癌症生物学和治疗潜力相关的发现.

主要成果:

  • RNase P参与染色体组合. 在染色体组合中,RNase P参与染色体组合.
  • RNase P参与了DNA损伤反应途径.
  • RNase P的失调与基因组不稳定性和癌症有关.

结论:

  • 在基因组维护中,RNase P具有关键的非正规功能.
  • 在瘤发生过程中RNase P的参与突显了它在癌症中的重要性.
  • 对于瘤治疗,RNase P 是一个有前途的治疗标.