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In-vitro Mutagenesis01:16

In-vitro Mutagenesis

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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相关实验视频

Updated: Jun 24, 2025

Recombineering Homologous Recombination Constructs in Drosophila
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Recombineering Homologous Recombination Constructs in Drosophila

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Red2Flpe-SCON:一种多功能,多色的策略,用于生成马赛克条件淘汰赛小鼠.

Szu-Hsien Sam Wu1,2, Somi Kim3, Heetak Lee4

  • 1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), Dr. Bohr-Gasse 3, 1030, Vienna, Austria.

Nature communications
|June 11, 2024
PubMed
概括

一个新的Red2Flpe-SCON小鼠模型允许对突变细胞和野生类型细胞进行多色谱系追踪. 这个系统揭示了Sox2基因对干细胞增殖和分化至关重要,而不是维护.

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相关实验视频

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科学领域:

  • 发展生物学 发展生物学
  • 遗传学 是一个遗传学.
  • 干细胞生物学 干细胞生物学

背景情况:

  • 基于图像的谱系追踪对于研究组织动态和细胞潜能至关重要.
  • 之前的Red2Onco模型促进了瘤性克隆分析.
  • 需要系统可以同时跟踪突变细胞和野生类型细胞.

研究的目的:

  • 开发一种新的马赛克淘汰系统,用于多色谱系追踪.
  • 使用这种新系统,研究Sox2在成年干细胞功能中的作用.

主要方法:

  • 开发Red2Flpe小鼠系列用于克隆特定的Flpe表达.
  • 实现基于FRT的SCON (Short Conditional IntrON) 方法用于可调调的马赛克淘汰赛.
  • 应用Red2Flpe-SCON系统用于Sox2突变细胞在小鼠食道上皮的克隆分析.

主要成果:

  • 红2Flpe-SCON系统能够对突变细胞和野生类型细胞进行多色标记.
  • 在食道上皮的Sox2突变克隆分析成功进行.
  • 发现Sox2对干细胞的维护不那么关键,但对增殖和分化至关重要.

结论:

  • 红2Flpe-SCON系统是一种强大的工具,用于体内马赛克分析.
  • Sox2在调节成年干细胞的增殖和分化方面发挥着至关重要的作用.
  • 这项研究完善了我们对干细胞基因功能在组织平衡中的理解.